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Literature summary for 3.4.21.35 extracted from

  • Lövgren, J.; Airas, K.; Lilja, H.
    Enzymatic action of human glandular kallikrein 2 (hK2). Substrate specificity and regulation by Zn2+ and extracellular protease inhibitors (1999), Eur. J. Biochem., 262, 781-789.
    View publication on PubMed
Search for more literature for 3.4.21.35

Inhibitors

Inhibitors Comment Organism Structure
antipain weak Homo sapiens
leupeptin weak Homo sapiens
Protein C inhibitor
-
 Homo sapiens
Zn2+
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 Ala-Arg-Arg-7-amido-4-methylcoumarin pH 7.5, 37°C Homo sapiens
0.04
-
 Pro-Phe-Arg-Arg-7-amido-4-methylcoumarin pH 7.5, 37°C Homo sapiens
0.048
-
 Val-Pro-Arg-Arg-7-amido-4-methylcoumarin pH 7.5, 37°C Homo sapiens
0.071
-
 Leu-Leu-Arg-7-amido-4-methylcoumarin pH 7.5, 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens likely the physiological activator of the zymogen form of prostate-specific antigen in the prostate ?
-
 ?
polypeptide + H2O Homo sapiens
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
prostate gland
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Ala-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?
Ala-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Ala-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
Fibronectin + H2O
-
 Homo sapiens ?
-
 ?
Leu-Leu-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Leu-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
additional information
-
 Homo sapiens ?
-
 ?
additional information likely the physiological activator of the zymogen form of prostate-specific antigen in the prostate Homo sapiens ?
-
 ?
polypeptide + H2O
-
 Homo sapiens peptides
-
 ?
Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
Pro-Phe-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Pro-Phe-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
pro-urokinase + H2O
-
 Homo sapiens urokinase
-
 ?
semenogelin I + H2O
-
 Homo sapiens semenogelin I fragments
-
 ?
semenogelin II + H2O
-
 Homo sapiens semenogelin II fragments
-
 ?
Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Val-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
Val-Pro-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Val-Pro-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
zymogen of prostate-specific antigen + H2O
-
 Homo sapiens prostate-specific antigen + APLILSR
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0267
-
 Val-Pro-Arg-Arg-7-amido-4-methylcoumarin pH 7.5, 37°C Homo sapiens
0.04
-
 Leu-Leu-Arg-7-amido-4-methylcoumarin pH 7.5, 37°C Homo sapiens
0.12
-
 Ala-Arg-Arg-7-amido-4-methylcoumarin pH 7.5, 37°C Homo sapiens
0.917
-
 Pro-Phe-Arg-Arg-7-amido-4-methylcoumarin pH 7.5, 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens
8
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 pH 6.5: about 30% of maximal activity, pH 9.5: about 40% of maximal activity Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000000025
-
 Protein C inhibitor pH 7.5, 37°C Homo sapiens