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Literature summary for 3.4.21.35 extracted from

  • Wang, J.; Chao, J.; Chao, L.
    Purification and characterization of recombinant tissue kallikrein from Escherichia coli and yeast (1991), Biochem. J., 276, 63-71.
No PubMed abstract available
Search for more literature for 3.4.21.35

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and in yeast Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
antipain wild-type and recombinant enzyme Rattus norvegicus
Aprotinin wild-type and recombinant enzyme Rattus norvegicus
leupeptin wild-type and recombinant enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polypeptide + H2O Rattus norvegicus
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
kininogen + H2O wild-type and recombinant enzyme Rattus norvegicus kinin + ?
-
 ?
low-molecular-weight kininogen + H2O
-
 Rattus norvegicus kallidin + kinin
-
 ?
polypeptide + H2O
-
 Rattus norvegicus peptides
-
 ?
Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Rattus norvegicus Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
Tosyl-Arg methyl ester + H2O
-
 Rattus norvegicus Tosyl-Arg + methanol
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 tosyl-Arg methyl ester hydrolase activity and kininogenase activity of wild-type and recombinant enzyme Rattus norvegicus