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Literature summary for 3.4.21.32 extracted from
- Purification, kinetical and molecular characterizations of a serine collagenolytic protease from greenshore crag (Carcinus maenas) digestive gland (1996), Comp. Biochem. Physiol. B, 115, 87-95.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3,4-dichloro-isocoumarin | - |
Carcinus maenas |  |
| alpha1 protease inhibitor | - |
Carcinus maenas | |
| Elastatinal | - |
Carcinus maenas | |
| Hg2+ | - |
Carcinus maenas | |
| Phenylmethylsulfonylfluoride | - |
Carcinus maenas | |
| Soybean trypsin inhibitor | - |
Carcinus maenas | |
| Zn2+ | - |
Carcinus maenas | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates | Carcinus maenas | |
| Mg2+ | activates | Carcinus maenas | |
| Mn2+ | activates | Carcinus maenas | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
x * 23000, SDS-PAGE | Carcinus maenas |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Carcinus maenas | - |
greenshore crab | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Carcinus maenas |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| digestive gland | - |
Carcinus maenas | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| room temperature, over one month | Carcinus maenas |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Collagen + H2O | type I and III | Carcinus maenas | Hydrolyzed collagen | - |
? | |
| succinyl-Ala-p-nitroanilide + H2O | - |
Carcinus maenas | ? | - |
? | |
| succinyl-L-Ala-L-Ala-L-Pro-L-Leu-p-nitroanilide + H2O | - |
Carcinus maenas | ? | - |
? | |
| succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide + H2O | - |
Carcinus maenas | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 23000, SDS-PAGE | Carcinus maenas |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Carcinus maenas |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Carcinus maenas |
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