Protein Variants | Comment | Organism |
---|---|---|
additional information | FXIa/PKA4 is made by replacing the FXI A4 domain with the A4 domain from prekallikrein. A dimeric version of FXI/PKA4 (FXI/PKA4-Gly326) is prepared as a control. Activated FXIa/PKA4 and FXIa/PKA4-Gly326 activate factor IX with kinetic parameters similar to that of FXIa. FXI/PKA4 and FXIa/PKA4-Gly326 have coagulant activity similar to FXI. FXIa/PKA4, FXIa/PKA4-Gly326 and FXIa have similar affinities for activated platelets. The dimeric proteins FXI and FXI/PKA4-Gly326 promote coagulation similarly, however, monomeric FXIa/PKA4 has greatly reduced activity. The activated monomeric FXIa/PKA4 activates factor IX poorly in the presence of activated platelets | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00012 | - |
factor IX | FXIa/PKA4, 37°C | Homo sapiens | |
0.00016 | - |
factor IX | FXIa/PKA4-Gly326, 37°C | Homo sapiens | |
0.0002 | - |
factor IX | factor FXIa, 37°C | Homo sapiens | |
0.45 | - |
L-pyroglutamyl-L-Pro-L-Arg-4-nitroanilide | FXIa/PKA4 | Homo sapiens | |
0.5 | - |
L-pyroglutamyl-L-Pro-L-Arg-4-nitroanilide | factor FXIa | Homo sapiens | |
0.59 | - |
L-pyroglutamyl-L-Pro-L-Arg-4-nitroanilide | FXIa/PKA4-Gly326 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
factor IX + H2O | model for factor IX activation in which FXIa binds to activated platelets by one chain of the dimer, while binding to factor IX through the other | Homo sapiens | activated factor IX + ? | - |
? | |
L-pyroglutamyl-L-Pro-L-Arg-4-nitroanilide + H2O | - |
Homo sapiens | L-pyroglutamyl-L-Pro-L-Arg + 4-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FXIa | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.4 | - |
factor IX | FXIa/PKA4, 37°C | Homo sapiens | |
5.7 | - |
factor IX | FXIa/PKA4-Gly326, 37°C | Homo sapiens | |
5.8 | - |
factor IX | factor FXIa, 37°C | Homo sapiens | |
250 | - |
L-pyroglutamyl-L-Pro-L-Arg-4-nitroanilide | FXIa/PKA4 | Homo sapiens | |
310 | - |
L-pyroglutamyl-L-Pro-L-Arg-4-nitroanilide | factor FXIa | Homo sapiens | |
430 | - |
L-pyroglutamyl-L-Pro-L-Arg-4-nitroanilide | FXIa/PKA4-Gly326 | Homo sapiens |