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Literature summary for 3.4.21.26 extracted from
- Post-proline cleaving enzyme from lamb brain (1981), J. Biochem., 90, 325-334.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-Chloromercuriphenylsulfonate | weak | Ovis aries |  |
| Chloromethylketone derivatives | - |
Ovis aries | |
| Cu2+ | - |
Ovis aries | |
| diisopropylfluorophosphate | - |
Ovis aries | |
| Hg2+ | - |
Ovis aries | |
| iodoacetate | weak | Ovis aries | |
| p-chloromercuribenzoate | - |
Ovis aries | |
| Pancreatic basic trypsin inhibitor | - |
Ovis aries |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Ovis aries | |
| 0.48 | - |
N-benzyloxycarbonyl-Gly-Pro-Ala | - |
Ovis aries | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | 74000 | gel filtration | Ovis aries |
| 77000 | - |
? * 77000, SDS-PAGE | Ovis aries |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ovis aries | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ovis aries |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Ovis aries |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 50% loss of activity after 1 week | Ovis aries |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzyloxycarbonyl-glycyl-proline-p-nitroanilide + H2O | - |
Ovis aries | ? | - |
? | |
| N-benzyloxycarbonyl-Gly-Pro-Ala + H2O | - |
Ovis aries | ? | - |
? | |
| Nalpha-benzyloxycarbonyl-Gly-Pro-Leu-Gly + H2O | - |
Ovis aries | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | ? * 77000, SDS-PAGE | Ovis aries |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Ovis aries |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 25 | 12 h, 50% loss of activity | Ovis aries |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Ovis aries | |
| 6.1 | - |
N-benzyloxycarbonyl-Gly-Pro-Ala | - |
Ovis aries | |
| 32.3 | - |
N-benzyloxycarbonyl-Gly-Pro-Leu-Gly | - |
Ovis aries | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Ovis aries |
| 7.2 | 7.5 | benzyloxycarbonylglycyl-proline-p-nitroanilide | Ovis aries |
| 7.8 | 8 | benzyloxycarbonylglycycl-prolyl-leucyl-glycinamide, benzyloxycarbonyl-glycyl-proline beta-naphthylamide | Ovis aries |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | 30°C, 1 h | Ovis aries |
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