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Literature summary for 3.4.21.26 extracted from

  • Kocadag Kocazorbaz, E.; Zihnioglu, F.
    Purification, characterization and the use of recombinant prolyl oligopeptidase from Myxococcus xanthus for gluten hydrolysis (2017), Protein Expr. Purif., 129, 101-107 .
    View publication on PubMed
Search for more literature for 3.4.21.26

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Myxococcus xanthus

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 48% residual activity at 10 mM Myxococcus xanthus
Cd2+ complete inhibition at 0.05 mM Myxococcus xanthus
Cu2+ 57% residual activity at 1 mM Myxococcus xanthus
dithiothreitol 86% residual activity at 5 mM Myxococcus xanthus
EDTA 94% residual activity at 1 mM Myxococcus xanthus
Hg2+ complete inhibition at 0.05 mM Myxococcus xanthus
K+ 63% residual activity at 1 mM Myxococcus xanthus
Mg2+ 87% residual activity at 1 mM Myxococcus xanthus
Mn2+ 87% residual activity at 1 mM Myxococcus xanthus
additional information not inhibited by p-aminobenzamidine, 1,10-phenanthroline and 2-mercaptoethanol Myxococcus xanthus
Ni2+ 58% residual activity at 0.05 mM Myxococcus xanthus
phenylmethylsulfonyl fluoride 18% residual activity at 1 mM Myxococcus xanthus
Z-Pro-prolinal 30% residual activity at 0.1 mM Myxococcus xanthus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 succinyl-L-Ala-L-Pro-4-nitroanilide at pH 7.5 and 37°C Myxococcus xanthus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Myxococcus xanthus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not influenced by Na+, Li2+ and Ca2+ Myxococcus xanthus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
wheat gluten + H2O Myxococcus xanthus
-
 ?
-
 ?
wheat gluten + H2O Myxococcus xanthus ATCC 25232
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Myxococcus xanthus
-
-
-
Myxococcus xanthus ATCC 25232
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity column chromatography and Ultragel AcA 44 gel filtration Myxococcus xanthus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8
-
 homogenate, at pH 7.5 and 37°C Myxococcus xanthus
226
-
 60.3fold purified enzyme, at pH 7.5 and 37°C Myxococcus xanthus

Storage Stability

Storage Stability Organism
4°C, recombinant enzyme, 6 weeks, 50% loss of activity Myxococcus xanthus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
IGF-1 + H2O H-Gly-Pro-Glu-OH Myxococcus xanthus ?
-
 ?
IGF-1 + H2O H-Gly-Pro-Glu-OH Myxococcus xanthus ATCC 25232 ?
-
 ?
additional information no activity with Ala-Pro-4-nitroanilide, Arg-Pro-4-nitroanilide, Val-Ala-4-nitroanilide, Gly-Pro-4-nitroanilide, Leu-4-nitroanilide, Pro-4-nitroanilide, Glu-4-nitroanilide, and Glu-Ala-4-nitroanilide Myxococcus xanthus ?
-
 ?
additional information no activity with Ala-Pro-4-nitroanilide, Arg-Pro-4-nitroanilide, Val-Ala-4-nitroanilide, Gly-Pro-4-nitroanilide, Leu-4-nitroanilide, Pro-4-nitroanilide, Glu-4-nitroanilide, and Glu-Ala-4-nitroanilide Myxococcus xanthus ATCC 25232 ?
-
 ?
Substance P + H2O Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 Myxococcus xanthus ?
-
 ?
Substance P + H2O Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 Myxococcus xanthus ATCC 25232 ?
-
 ?
succinyl-Gly-L-Pro-4-nitroanilide + H2O
-
 Myxococcus xanthus succinyl-Gly-L-Pro + 4-nitroaniline
-
 ?
succinyl-L-Ala-L-Pro-4-nitroanilide + H2O
-
 Myxococcus xanthus succinyl-L-Ala-L-Pro + 4-nitroaniline
-
 ?
succinyl-L-Arg-L-Pro-4-nitroanilide + H2O
-
 Myxococcus xanthus succinyl-L-Arg-L-Pro + 4-nitroaniline
-
 ?
wheat gluten + H2O
-
 Myxococcus xanthus ?
-
 ?
wheat gluten + H2O
-
 Myxococcus xanthus ATCC 25232 ?
-
 ?
Z-Gly-L-Pro-4-nitroanilide + H2O highest activity Myxococcus xanthus Z-Gly-L-Pro + 4-nitroaniline
-
 ?
Z-Gly-L-Pro-4-nitroanilide + H2O highest activity Myxococcus xanthus ATCC 25232 Z-Gly-L-Pro + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Myxococcus xanthus

Synonyms

Synonyms Comment Organism
POP
-
 Myxococcus xanthus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Myxococcus xanthus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 37 the purified enzyme is stable over broad range of temperature (4-37°C) retaining 100% activity and retaining 50% activity at 50°C Myxococcus xanthus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Myxococcus xanthus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8.5 the purified enzyme is stable in a pH range of 6.0-8.5 Myxococcus xanthus

pI Value

Organism Comment pI Value Maximum pI Value
Myxococcus xanthus isoelectric focusing
-
 6.3