Cloned (Comment) | Organism |
---|---|
gene PREP, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2S)-1-[[(2S)-1-(1-oxo-4-phenylbutyl)-2-pyrrolidinyl]carbonyl]-2-pyrrolidinecarbonitrile | i.e. KYP-2047, hyperbolic kinetics of KYP-2047 binding to human PREP with pseudo-firstorder rate constant, Van't Hoff plot for binding of KYP-2047 to human PREP, a covalent bond is formed between the nitrile group of KYP-2047 and the active site serine | Homo sapiens | |
(2S)-1-[[(2S)-1-(1-oxo-4-phenylbutyl)-2-pyrrolidinyl]carbonyl]-2-pyrrolidinecarbonitrile | i.e. KYP-2047 | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics and thermodynamics, comparison of human and porcine enzymes, human and porcine PREP have a similar kinetic behavior, overview | Sus scrofa | |
additional information | - |
additional information | steady-state kinetics and thermodynamics, comparison of human and porcine enzymes, human and porcine PREP have a similar kinetic behavior, overview | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
bradykinin + H2O | Sus scrofa | - |
? | - |
? | |
bradykinin + H2O | Homo sapiens | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P48147 | - |
- |
Sus scrofa | P23687 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and anion exchange chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bradykinin + H2O | - |
Sus scrofa | ? | - |
? | |
bradykinin + H2O | - |
Homo sapiens | ? | - |
? | |
Gly-Pro-4-nitrophenyl ester + H2O | - |
Sus scrofa | Gly-Pro + 4-nitrophenol | - |
? | |
Gly-Pro-4-nitrophenyl ester + H2O | - |
Homo sapiens | Gly-Pro + 4-nitrophenol | - |
? | |
N-benzyloxycarbonyl-Gly-Pro-4-nitrophenyl ester + H2O | - |
Sus scrofa | N-benzyloxycarbonyl-Gly-Pro + 4-nitrophenol | - |
? | |
N-benzyloxycarbonyl-Gly-Pro-4-nitrophenyl ester + H2O | - |
Homo sapiens | N-benzyloxycarbonyl-Gly-Pro + 4-nitrophenol | - |
? | |
succinyl-Ala-Pro-4-nitrophenyl ester + H2O | - |
Sus scrofa | succinyl-Ala-Pro + 4-nitrophenol | - |
? | |
succinyl-Ala-Pro-4-nitrophenyl ester + H2O | - |
Homo sapiens | succinyl-Ala-Pro + 4-nitrophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PREP | - |
Sus scrofa |
PREP | - |
Homo sapiens |
prolyl oligopeptidase | - |
Sus scrofa |
prolyl oligopeptidase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Sus scrofa |
7.5 | 8 | recombinant enzyme | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000001 | - |
(2S)-1-[[(2S)-1-(1-oxo-4-phenylbutyl)-2-pyrrolidinyl]carbonyl]-2-pyrrolidinecarbonitrile | pH 7.5, 37°C, recombinant enzyme | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | human PREP behaves identically to the porcine homologue, displaying a double bell-shaped pH profile and a pH-dependent solvent kinetic isotope effect of the kcat/Km, features that set it apart from the related exopeptidase dipeptidyl peptidase IV (DPP IV) | Sus scrofa |
additional information | human PREP behaves identically to the porcine homologue, displaying a double bell-shaped pH profile and a pH-dependent solvent kinetic isotope effect of the kcat/Km, features that set it apart from the related exopeptidase dipeptidyl peptidase IV (DPP IV) | Homo sapiens |