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Literature summary for 3.4.21.26 extracted from

  • Chen, J.T.; Chao, M.L.; Wen, C.Y.; Chu, W.S.
    Screening, purification, and characterization of an extracellular prolyl oligopeptidase from Coprinopsis clastophylla (2012), J. Microbiol., 50, 652-659.
    View publication on PubMed
Search for more literature for 3.4.21.26

Activating Compound

Activating Compound Comment Organism Structure
3,4-dichloroisocoumarin
-
 Coprinopsis clastophylla

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate 14% residual activity at 0.2 mM Coprinopsis clastophylla
4-toluenesulfonamide 98% residual activity at 0.2 mM Coprinopsis clastophylla
Co2+ 48% residual activity at 0.2 mM Coprinopsis clastophylla
Cu2+ 13% residual activity at 0.2 mM Coprinopsis clastophylla
diisopropyl fluorophosphate 13% residual activity at 0.2 mM Coprinopsis clastophylla
EDTA 93% residual activity at 0.2 mM Coprinopsis clastophylla
Hg2+ complete inhibition at 0.2 mM Coprinopsis clastophylla
iodoacetamide 87% residual activity at 0.2 mM Coprinopsis clastophylla
Mn2+ 93% residual activity at 0.2 mM Coprinopsis clastophylla
N-ethylmaleimide 50% residual activity at 0.2 mM Coprinopsis clastophylla
Ni2+ 86% residual activity at 0.2 mM Coprinopsis clastophylla
o-phenanthroline 79% residual activity at 0.2 mM Coprinopsis clastophylla
Sn2+ 87% residual activity at 0.2 mM Coprinopsis clastophylla
Zn2+ 61% residual activity at 0.2 mM Coprinopsis clastophylla

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.74
-
 benzyloxycarbonyl-Gly-L-Pro-7-amido-4-methylcoumarin at pH 8.0 and 37°C Coprinopsis clastophylla
3.42
-
 benzyloxycarbonyl-Gly-L-Pro-4-nitroanilide at pH 8.0 and 37°C Coprinopsis clastophylla

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ activates Coprinopsis clastophylla

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84000
-
 x * 84000, SDS-PAGE Coprinopsis clastophylla

Organism

Organism UniProt Comment Textmining
Coprinopsis clastophylla
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, phenyl Sepharose column chromatography, Sephacryl S-200 gel filtration, hydroxyapatite column chromatography, and Resource Q column chromatography Coprinopsis clastophylla

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.18
-
 supernatant, using benzyloxycarbonyl-Gly-L-Pro-4-nitroanilide as substrate, at pH 8.0 and 37°C Coprinopsis clastophylla
52.1
-
 after 285fold purification, using benzyloxycarbonyl-Gly-L-Pro-4-nitroanilide as substrate, at pH 8.0 and 37°C Coprinopsis clastophylla
61.3
-
 after 285fold purification, using benzyloxycarbonyl-Gly-L-Pro-7-amido-4-methylcoumarin as substrate, at pH 8.0 and 37°C Coprinopsis clastophylla

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Gly-L-Pro-4-nitroanilide + H2O
-
 Coprinopsis clastophylla benzyloxycarbonyl-Gly-L-Pro + 4-nitroaniline
-
 ?
benzyloxycarbonyl-Gly-L-Pro-7-amido-4-methylcoumarin + H2O
-
 Coprinopsis clastophylla benzyloxycarbonyl-Gly-L-Pro + 7-amino-4-methylcoumarin
-
 ?
additional information no activity toward L-Pro-4-nitroanilide, Gly-L-Pro-4-nitroanilide, Z-Pro-nitrophenyl ester, Z-Gly-Gly-nitrophenyl ester, Z-Phe-Arg-7-amido-4-methylcoumarin, Z-Leu-Leu-Glu-7-amido-4-methylcoumarin, Z-Val-Lys-Met-7-amido-4-methylcoumarin, Z-Leu-Leu-Leu-7-amido-4-methylcoumarin, and Z-Tyr-Val-Ala-Asp-4-nitroanilide Coprinopsis clastophylla ?
-
 ?

Subunits

Subunits Comment Organism
? x * 84000, SDS-PAGE Coprinopsis clastophylla

Synonyms

Synonyms Comment Organism
post-proline cleaving endopeptidase
-
 Coprinopsis clastophylla
proline-specific endopeptidase
-
 Coprinopsis clastophylla
prolyl endopeptidase
-
 Coprinopsis clastophylla

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Coprinopsis clastophylla

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 37 the enzyme is active over a temperature range of 25-37°C Coprinopsis clastophylla

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 the enzyme retains about 20% of its activity after incubation at 45°C for 30 min Coprinopsis clastophylla

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
26.6
-
 benzyloxycarbonyl-Gly-L-Pro-7-amido-4-methylcoumarin at pH 8.0 and 37°C Coprinopsis clastophylla
162
-
 benzyloxycarbonyl-Gly-L-Pro-4-nitroanilide at pH 8.0 and 37°C Coprinopsis clastophylla

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Coprinopsis clastophylla

pH Range

pH Minimum pH Maximum Comment Organism
6 9 the activity of the enzyme is reduced by 50% and 60% at pH 6.0 and 9.0, respectively, compared to its maximal activity Coprinopsis clastophylla

pI Value

Organism Comment pI Value Maximum pI Value
Coprinopsis clastophylla isoelectric focusing
-
 5.2

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.2
-
 at pH 8.0 and 37°C Coprinopsis clastophylla N-ethylmaleimide

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
15.1
-
 benzyloxycarbonyl-Gly-L-Pro-7-amido-4-methylcoumarin at pH 8.0 and 37°C Coprinopsis clastophylla
47.4
-
 benzyloxycarbonyl-Gly-L-Pro-4-nitroanilide at pH 8.0 and 37°C Coprinopsis clastophylla