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Literature summary for 3.4.21.26 extracted from

  • Huang, C.H.; Suen, C.S.; Lin, C.T.; Chien, C.H.; Lee, H.Y.; Chung, K.M.; Tsai, T.Y.; Jiaang, W.T.; Hwang, M.J.; Chen, X.
    Cleavage-site specificity of prolyl endopeptidase FAP investigated with a full-length protein substrate (2011), J. Biochem., 149, 685-692.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Hi5 insect cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
(2S)-1-({(2S,4S)-4-[2-(1,3-Dihydro-2H-isoindol-2-yl)-2-oxo- ethyl]-5-oxopyrrolidin-2-yl}carbonyl)-4,4-difluoropyrrolidine-2- carbonitrile potent and selective inhibitor of FAP, 0.01 mM used in assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpa2-antiplasmin + H2O Homo sapiens not a robust substrate in vitro, the enzyme cleaves after Pro12 in the T9S10G11P12-N13 Q14E15Q16E17 sequence ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Ni-Sepharose resin column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpa2-antiplasmin + H2O not a robust substrate in vitro, the enzyme cleaves after Pro12 in the T9S10G11P12-N13 Q14E15Q16E17 sequence Homo sapiens ?
-
?
additional information fibroblast activation protein prefers uncharged residues, including small or bulky hydrophobic amino acids, but not charged amino acids, especially acidic residue at P1Ā’, P3 and P4 sites. Fibroblast activation protein cannot cleave interleukins Homo sapiens ?
-
?
SPRY2 + H2O not an in vivo substrate of fibroblast activation protein Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
FAP
-
Homo sapiens
fibroblast activation protein
-
Homo sapiens
prolyl endopeptidase
-
Homo sapiens