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Literature summary for 3.4.21.26 extracted from

  • Szeltner, Z.; Rea, D.; Juhasz, T.; Renner, V.; Mucsi, Z.; Orosz, G.; Fulop, V.; Polgar, L.
    Substrate-dependent competency of the catalytic triad of prolyl oligopeptidase (2002), J. Biol. Chem., 277, 44597-44605.
    View publication on PubMed
Search for more literature for 3.4.21.26

Crystallization (Commentary)

Crystallization (Comment) Organism
D641A and D641N mutants are cocrystallized in presence of substrate benzyloxycarbonyl-Gly-Pro-4-nitroanilide Sus scrofa

Protein Variants

Protein Variants Comment Organism
D641A the ratio of turnover number to Km-value for the substrate benzyloxycarbonyl-Gly-Pro-beta-naphthylamide is 0.2% of the wild-type ratio. The ratio of turnover number to Km-value for the substrate 2-aminobenzoyl-Gly-Phe-Gly-Pro-Phe-Gly-Phe(NO2)-Ala-NH2 is 1.8fold higher than the wild-type ratio. The ratio of turnover number to Km-value for the substrate benzyloxycarbonyl-Gly-Pro-p-nitrophenol is 66% of the wild-type ratio. The ratio of turnover number to Km-value for the substrate benzyloxycarbonyl-Gly-Pro-SBzl is 0.92% of the wild-type ratio Sus scrofa
D641N the ratio of turnover number to Km-value for the substrate benzyloxycarbonyl-Gly-Pro-beta-naphthylamide is 0.02% of the wild-type ratio. The ratio of turnover number to Km-value for the substrate 2-aminobenzoyl-Gly-Phe-Gly-Pro-Phe-Gly-Phe(NO2)-Ala-NH2 is 1.4fold higher than the wild-type ratio. The ratio of turnover number to Km-value for the substrate benzyloxycarbonyl-Gly-Pro-p-nitrophenol is 40% of the wild-type ratio. The ratio of turnover number to Km-value for the substrate benzyloxycarbonyl-Gly-Pro-SBzl is 0.27% of the wild-type ratio Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
benzyloxycarbonyl-Gly-Pro-CH2Cl
-
 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000025
-
 benzyloxycarbonyl-Gly-Pro-p-nitrophenol pH 7.0, mutant enzyme D641A Sus scrofa
0.000028
-
 benzyloxycarbonyl-Gly-Pro-p-nitrophenol pH 7.0, mutant enzyme D641N Sus scrofa
0.00152
-
 benzyloxycarbonyl-Gly-Pro-SBzl pH 7.0, mutant enzyme D641N Sus scrofa
0.00159
-
 benzyloxycarbonyl-Gly-Pro-SBzl pH 7.0, mutant enzymeD641A Sus scrofa
0.00205
-
 2-aminobenzoyl-Gly-Phe-Gly-Pro-Phe-Gly-Phe(NO2)-Ala-NH2 pH 8.0, wild-type enzyme Sus scrofa
0.0054
-
 benzyloxycarbonyl-Gly-Pro-beta-naphthylamide pH 7.0, mutant enzyme D641A Sus scrofa
0.0059
-
 benzyloxycarbonyl-Gly-Pro-beta-naphthylamide pH 7.0, wild-type enzyme Sus scrofa
0.0135
-
 benzyloxycarbonyl-Gly-Pro-beta-naphthylamide pH 7.0, mutant enzyme D641N Sus scrofa
0.016
-
 benzyloxycarbonyl-Gly-Pro-SBzl pH 7.0, wild-type enzyme Sus scrofa
0.0192
-
 benzyloxycarbonyl-Gly-Pro-p-nitrophenyl ester pH 7.0, wild-type enzyme Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa P23687
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-Gly-Phe-Gly-Pro-Phe-Gly-Phe(NO2)-Ala-NH2 + H2O
-
 Sus scrofa 2-aminobenzoyl-Gly-Phe-Gly-Pro + Phe-Gly-Phe(NO2)-Ala-NH2
-
 ?
benzyloxycarbonyl-Gly-Pro-beta-naphthylamide + H2O
-
 Sus scrofa benzyloxycarbonyl-Gly-Pro + 2-naphthylamine
-
 ?
benzyloxycarbonyl-Gly-Pro-p-nitrophenol + H2O
-
 Sus scrofa benzyloxycarbonyl-Gly-Pro + p-nitrophenol
-
 ?
benzyloxycarbonyl-Gly-Pro-SBzl + H2O
-
 Sus scrofa benzyloxycarbonyl-Gly-Pro + phenyl-methanethiol
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0151
-
 benzyloxycarbonyl-Gly-Pro-beta-naphthylamide pH 7.0, mutant enzyme D641N Sus scrofa
0.021
-
 benzyloxycarbonyl-Gly-Pro-p-nitrophenol pH 7.0, mutant enzyme D641N Sus scrofa
0.028
-
 benzyloxycarbonyl-Gly-Pro-SBzl pH 7.0, mutante enzyme D641N Sus scrofa
0.031
-
 benzyloxycarbonyl-Gly-Pro-p-nitrophenol pH 7.0, mutant enzyme D641A Sus scrofa
0.0657
-
 benzyloxycarbonyl-Gly-Pro-beta-naphthylamide pH 7.0, mutant enzyme D641A Sus scrofa
0.099
-
 benzyloxycarbonyl-Gly-Pro-SBzl pH 7.0, mutant enzymeD641A Sus scrofa
3 6 benzyloxycarbonyl-Gly-Pro-p-nitrophenol pH 7.0, wild-type enzyme Sus scrofa
3.3
-
 2-aminobenzoyl-Gly-Phe-Gly-Pro-Phe-Gly-Phe(NO2)-Ala-NH2 pH 8.0, wild-type enzyme Sus scrofa
5.38
-
 2-aminobenzoyl-Gly-Phe-Gly-Pro-Phe-Gly-Phe(NO2)-Ala-NH2 pH 8.0, wild-type enzyme Sus scrofa
32.5
-
 benzyloxycarbonyl-Gly-Pro-beta-naphthylamide pH 7.0, wild-type enzyme Sus scrofa
108
-
 benzyloxycarbonyl-Gly-Pro-SBzl pH 7.0, wild-type enzyme Sus scrofa