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Literature summary for 3.4.21.25 extracted from

  • Uchikoba, T.; Yonezawa, H.; Kaneda, M.
    Cleavage specificity of cucumisin, a plant serine protease (1995), J. Biochem., 117, 1126-1130.
    View publication on PubMed
Search for more literature for 3.4.21.25

General Stability

General Stability Organism
the stability of the 67000 Da and the 54000 Da enzyme is almost the same Cucumis melo

Inhibitors

Inhibitors Comment Organism Structure
alpha2-Macroglobulin 38% inhibition by a concentration of 0.001 mM with casein as substrate, and 18% inhibition with succinyl-Ala-Pro-Ala-p-nitroanilide as substrate Cucumis melo
diisopropyl fluorophosphate complete inhibition by 1 mM Cucumis melo
additional information no effect of leupeptin, alpha1-antitrypsin, soybean trypsin inhibitor, ovomucoid and aprotinin Cucumis melo

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
 Cucumis melo
1.25
-
 glutaryl-Ala-Ala-Pro-Leu-p-nitroanilide
-
 Cucumis melo
1.96
-
 succinyl-Ala-Pro-Ala-p-nitroanilide
-
 Cucumis melo
2.72
-
 succinyl-Ala-Ala-Ala-p-nitroanilide
-
 Cucumis melo
2.91
-
 succinyl-Ala-Ala-p-nitroanilide
-
 Cucumis melo
3.57
-
 succinyl-Ala-p-nitroanilide
-
 Cucumis melo

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 SDS-PAGE Cucumis melo

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Protein + H2O Cucumis melo
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Cucumis melo
-
 L. var Prince
-

Purification (Commentary)

Purification (Comment) Organism
a quick purification method leads to an enzyme fraction with almost only the 67000 Da enzyme Cucumis melo

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-mating factor + H2O cleaves at one site, Glu-Leu Cucumis melo ?
-
 ?
angiotensin I + H2O cleaves at two sites, Tyr-Ile and Phe-His Cucumis melo ?
-
 ?
casein + H2O
-
 Cucumis melo ?
-
 ?
Cytochrome c + H2O initial cleavage site, Glu-Lys and Ala-Ala Cucumis melo ?
-
 ?
delta sleep-inducing peptide + H2O cleaves at one site, Ala-Ser Cucumis melo ?
-
 ?
glutaryl-Ala-Ala-Pro-Leu-p-nitroanilide + H2O
-
 Cucumis melo ?
-
 ?
insulin + H2O initial cleavage site, Gln-His Cucumis melo ?
-
 ?
Insulin B-chain + H2O three cleavage sites are detected after a reaction period of 30 s, Leu-Cys, Cys-Gly, and Glu-Ala Cucumis melo ?
-
 ?
additional information the substrate specificity of the 67000 Da and the 54000 Da enzyme is almost the same Cucumis melo ?
-
 ?
Myoglobin + H2O initial cleavage site, Asp-Ile and Glu-Ala Cucumis melo ?
-
 ?
ovalbumin + H2O initial cleavage site, Asn-Ala Cucumis melo ?
-
 ?
Protein + H2O
-
 Cucumis melo ?
-
 ?
protein + H2O
-
 Cucumis melo hydrolyzed protein
-
 ?
succinyl-Ala-Ala-Ala-p-nitroanilide + H2O
-
 Cucumis melo ?
-
 ?
succinyl-Ala-Ala-p-nitroanilide + H2O
-
 Cucumis melo ?
-
 ?
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
-
 Cucumis melo ?
-
 ?
succinyl-Ala-p-nitroanilide + H2O
-
 Cucumis melo succinyl-Ala + 4-nitroaniline
-
 ?
succinyl-Ala-Pro-Ala-p-nitroanilide + H2O
-
 Cucumis melo ?
-
 ?
superoxide dismutase + H2O initial cleavage site, His-Gly and Leu-Ala Cucumis melo ?
-
 ?
TMA-lysozyme BrCN fragment + H2O cleaves at two sites, Cys-Lys and Arg-Gly Cucumis melo ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 60 fairly stable at pH 7.1 up to Cucumis melo

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
 succinyl-Ala-p-nitroanilide
-
 Cucumis melo
0.007
-
 succinyl-Ala-Ala-p-nitroanilide
-
 Cucumis melo
0.013
-
 succinyl-Ala-Ala-Ala-p-nitroanilide
-
 Cucumis melo
0.31
-
 succinyl-Ala-Pro-Ala-p-nitroanilide
-
 Cucumis melo
4.17
-
 succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
 Cucumis melo
5.9
-
 glutaryl-Ala-Ala-Pro-Leu-p-nitroanilide
-
 Cucumis melo

pH Stability

pH Stability pH Stability Maximum Comment Organism
11
-
 retains about 80% of the activity Cucumis melo