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Literature summary for 3.4.21.25 extracted from

  • Kaneda, M.; Yonezawa, H.; Uchikoba, T.
    Improved isolation, stability and substrate specificity of cucumisin, a plant serine endopeptidase (1995), Biotechnol. Appl. Biochem., 22, 215-222.
    View publication on PubMed
Search for more literature for 3.4.21.25

General Stability

General Stability Organism
20% dimethyl sulfoxide solution, 20°C, stable Cucumis melo
8 M urea, pH 9.1, 20 min, no loss of activity Cucumis melo
alpha-chymotrypsin has no effect on the activity Cucumis melo
incubation with trypsin for 4 h decreases the activity to 30% of the initial activity and after 24 h the enzyme is completely inactivated Cucumis melo

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.02
-
 Benzyloxycarbonyl-Glu-Tyr
-
 Cucumis melo
3.33
-
 benzoyl-Tyr-ethyl ester
-
 Cucumis melo

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 SDS-PAGE Cucumis melo

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Protein + H2O Cucumis melo
-
 ?
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
2-propanol 20% w/v, at 37°C for 1 h, no effect Cucumis melo
acetonitrile 20% w/v, at 37°C for 1 h, no effect Cucumis melo
dioxan 20% w/v, at 37°C for 1 h, no effect Cucumis melo
Ethanol 20% w/v, at 37°C for 1 h, no effect Cucumis melo

Organism

Organism UniProt Comment Textmining
Cucumis melo
-
-
-

Purification (Commentary)

Purification (Comment) Organism
40fold Cucumis melo

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-Ala-Ala-Ala-p-nitroanilide + H2O 1% of the activity with benzyloxycarbonyl-Tyr-nitrophenyl ester Cucumis melo ?
-
 ?
benzoyl-Tyr-ethyl ester + H2O 24% of the activity with benzyloxycarbonyl-Tyr-nitrophenyl ester Cucumis melo benzoyl-Tyr + ethanol
-
 ?
Benzyloxycarbonyl-Glu-Tyr + H2O
-
 Cucumis melo Benzyloxycarbonyl-Glu + Tyr
-
 ?
benzyloxycarbonyl-Tyr-4-nitrophenyl ester + H2O best synthetic substrate Cucumis melo benzyloxycarbonyl-Tyr + 4-nitrophenyl ester
-
 ?
casein + H2O
-
 Cucumis melo ?
-
 ?
Insulin B-chain + H2O broad substrate specificity, but the bonds of the valine residue are not split Cucumis melo ?
-
 ?
additional information no hydrolysis of Lys-p-nitroanilide, Tyr-p-nitroanilide, Leu-p-nitroanilide, tosyl-Arg-methyl ester, acetyl-Phe-ethyl ester, benzyloxycarbonyl-Lys-nitrophenyl ester and benzyloxycarbonyl-Gly-Gly-Leu-p-nitroanilide Cucumis melo ?
-
 ?
Protein + H2O
-
 Cucumis melo ?
-
 ?
protein + H2O
-
 Cucumis melo hydrolyzed protein
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 20 h at a pH of 11, 80% of the activity Cucumis melo
37
-
 24 h, 80% of the activity remains Cucumis melo
47
-
 20 min at a pH of 11, no loss of activity Cucumis melo
60
-
 1 h at a pH of 11, 65% of the activity Cucumis melo
72
-
 inactivated after 20 min, ethylene glycol does not stablize, glycerol is effective to some extent Cucumis melo

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.22
-
 benzoyl-Tyr-ethyl ester
-
 Cucumis melo
2.2
-
 benzyloxycarbonyl-Tyr 4-nitrophenyl ester
-
 Cucumis melo