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Literature summary for 3.4.21.25 extracted from

  • Yamagata, H.; Masuzawa, T.; Nagaoka, Y.; Ohnishi, T.; Iwasaki, T.
    Cucumisin, a serine protease from melon fruits, shares structural homology with subtilisin and is generated from a large precursor (1994), J. Biol. Chem., 269, 32725-32731.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned in Escherichia coli Y1090r- Cucumis melo

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
native enzyme, a large precursor has a MW of 67000 Da, consists of a 14000 Da C-terminal polypeptide and the active 54000 Da protease domain, which arises by limited autolysis, SDS-PAGE Cucumis melo

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Protein + H2O Cucumis melo
-
?
-
?

Organism

Organism UniProt Comment Textmining
Cucumis melo
-
L. var reticulatus cv. Teresa
-

Purification (Commentary)

Purification (Comment) Organism
-
Cucumis melo

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
Cucumis melo
-
juice
-
Cucumis melo
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Protein + H2O
-
Cucumis melo ?
-
?
protein + H2O
-
Cucumis melo hydrolyzed protein
-
?

Synonyms

Synonyms Comment Organism
More
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-