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Literature summary for 3.4.21.25 extracted from

  • Kaneda, M.; Tominaga, N.
    Properties of a new plant serine protease cucumisin (1987), Agric. Biol. Chem., 51, 489-492.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Protein + H2O Cucumis melo
-
?
-
?

Organism

Organism UniProt Comment Textmining
Cucumis melo
-
L. var Prince
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity of the immobilized enzyme is 41% of that of the soluble enzyme Cucumis melo

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
Cucumis melo ?
-
?
Protein + H2O
-
Cucumis melo ?
-
?
protein + H2O
-
Cucumis melo hydrolyzed protein
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
soluble enzyme Cucumis melo
85
-
immobilized enzyme Cucumis melo

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8 soluble enzyme Cucumis melo
9
-
immobilized enzyme Cucumis melo

pH Range

pH Minimum pH Maximum Comment Organism
3 11 pH 3: below 5% of the maximal activity, pH 11: 15% of the maximal activity Cucumis melo
4 12 pH 4: 50% of the maximal activity, pH 12: 10% of the maximal activity Cucumis melo

pH Stability

pH Stability pH Stability Maximum Comment Organism
11
-
1 h incubation at 60°C, the immobilized enzyme is more stable than the soluble, keeps 80% of the activity Cucumis melo