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Literature summary for 3.4.21.22 extracted from

  • Perot, E.; Enjolras, N.; Le Quellec, S.; Indalecio, A.; Girard, J.; Negrier, C.; Dargaud, Y.
    Expression and characterization of a novel human recombinant factor IX molecule with enhanced in vitro and in vivo clotting activity (2015), Thromb. Res., 135, 1017-1024 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Huh-7 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
E410A the mutant shows reduced hydrolytic activity and increased in-vitro clotting activity compared to the wild type enzyme Homo sapiens
E410H the mutant with reduced hydrolytic activity and increased in-vitro clotting activity has 2.5fold enhanced affinity for the cofactor FVIIIa and induces 5.2fold higher thrombin generation compared to the wild type enzyme Homo sapiens
E410L the mutant shows reduced hydrolytic activity and increased in-vitro clotting activity compared to the wild type enzyme Homo sapiens
E410N the mutant shows reduced hydrolytic activity and increased in-vitro clotting activity compared to the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00002877
-
Factor X wild type enzyme, in the presence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
0.00005793
-
Factor X mutant enzyme E410N, in the presence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
0.00006988
-
Factor X mutant enzyme E410L, in the presence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
0.0000751
-
Factor X mutant enzyme E410H, in the presence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
0.00008004
-
Factor X mutant enzyme E410A, in the presence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
0.0002227
-
Factor X mutant enzyme E410N, in the absence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
0.0002241
-
Factor X mutant enzyme E410A, in the absence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
0.000231
-
Factor X mutant enzyme E410H, in the absence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
0.0002328
-
Factor X mutant enzyme E410L, in the absence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
0.0004478
-
Factor X wild type enzyme, in the absence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Factor X + H2O Homo sapiens
-
Factor Xa + ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Q column chromatography and HiTrap heparin column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Factor X + H2O
-
Homo sapiens Factor Xa + ?
-
?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
activated FIX
-
Homo sapiens
FIXa
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00096
-
Factor X mutant enzyme E410H, in the absence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
0.00096
-
Factor X mutant enzyme E410N, in the absence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
0.00105
-
Factor X mutant enzyme E410A, in the absence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
0.0012
-
Factor X mutant enzyme E410L, in the absence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
0.00127
-
Factor X wild type enzyme, in the absence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
0.00157
-
Factor X wild type enzyme, in the presence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
0.00248
-
Factor X mutant enzyme E410N, in the presence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
0.00361
-
Factor X mutant enzyme E410L, in the presence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
0.00397
-
Factor X mutant enzyme E410H, in the presence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
0.00399
-
Factor X mutant enzyme E410A, in the presence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
Factor VIIIa
-
Homo sapiens

General Information

General Information Comment Organism
malfunction hemophilia B is an inherited X-linked recessive bleeding disorder, due to a defect in human factor IX Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.825
-
Factor X wild type enzyme, in the absence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
4.163
-
Factor X mutant enzyme E410H, in the absence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
4.326
-
Factor X mutant enzyme E410N, in the absence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
4.374
-
Factor X mutant enzyme E410L, in the absence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
4.693
-
Factor X mutant enzyme E410A, in the absence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
42800
-
Factor X mutant enzyme E410N, in the presence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
49800
-
Factor X mutant enzyme E410A, in the presence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
51600
-
Factor X mutant enzyme E410L, in the presence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
52900
-
Factor X mutant enzyme E410H, in the presence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens
54500
-
Factor X wild type enzyme, in the presence of factor VIIIa, at pH 7.4 and 37┬░C Homo sapiens