Cloned (Comment) | Organism |
---|---|
expressed in Huh-7 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E410A | the mutant shows reduced hydrolytic activity and increased in-vitro clotting activity compared to the wild type enzyme | Homo sapiens |
E410H | the mutant with reduced hydrolytic activity and increased in-vitro clotting activity has 2.5fold enhanced affinity for the cofactor FVIIIa and induces 5.2fold higher thrombin generation compared to the wild type enzyme | Homo sapiens |
E410L | the mutant shows reduced hydrolytic activity and increased in-vitro clotting activity compared to the wild type enzyme | Homo sapiens |
E410N | the mutant shows reduced hydrolytic activity and increased in-vitro clotting activity compared to the wild type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00002877 | - |
Factor X | wild type enzyme, in the presence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
0.00005793 | - |
Factor X | mutant enzyme E410N, in the presence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
0.00006988 | - |
Factor X | mutant enzyme E410L, in the presence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
0.0000751 | - |
Factor X | mutant enzyme E410H, in the presence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
0.00008004 | - |
Factor X | mutant enzyme E410A, in the presence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
0.0002227 | - |
Factor X | mutant enzyme E410N, in the absence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
0.0002241 | - |
Factor X | mutant enzyme E410A, in the absence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
0.000231 | - |
Factor X | mutant enzyme E410H, in the absence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
0.0002328 | - |
Factor X | mutant enzyme E410L, in the absence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
0.0004478 | - |
Factor X | wild type enzyme, in the absence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Factor X + H2O | Homo sapiens | - |
Factor Xa + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Q column chromatography and HiTrap heparin column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Factor X + H2O | - |
Homo sapiens | Factor Xa + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
activated FIX | - |
Homo sapiens |
FIXa | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00096 | - |
Factor X | mutant enzyme E410H, in the absence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
0.00096 | - |
Factor X | mutant enzyme E410N, in the absence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
0.00105 | - |
Factor X | mutant enzyme E410A, in the absence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
0.0012 | - |
Factor X | mutant enzyme E410L, in the absence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
0.00127 | - |
Factor X | wild type enzyme, in the absence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
0.00157 | - |
Factor X | wild type enzyme, in the presence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
0.00248 | - |
Factor X | mutant enzyme E410N, in the presence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
0.00361 | - |
Factor X | mutant enzyme E410L, in the presence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
0.00397 | - |
Factor X | mutant enzyme E410H, in the presence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
0.00399 | - |
Factor X | mutant enzyme E410A, in the presence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Factor VIIIa | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | hemophilia B is an inherited X-linked recessive bleeding disorder, due to a defect in human factor IX | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.825 | - |
Factor X | wild type enzyme, in the absence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
4.163 | - |
Factor X | mutant enzyme E410H, in the absence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
4.326 | - |
Factor X | mutant enzyme E410N, in the absence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
4.374 | - |
Factor X | mutant enzyme E410L, in the absence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
4.693 | - |
Factor X | mutant enzyme E410A, in the absence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
42800 | - |
Factor X | mutant enzyme E410N, in the presence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
49800 | - |
Factor X | mutant enzyme E410A, in the presence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
51600 | - |
Factor X | mutant enzyme E410L, in the presence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
52900 | - |
Factor X | mutant enzyme E410H, in the presence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens | |
54500 | - |
Factor X | wild type enzyme, in the presence of factor VIIIa, at pH 7.4 and 37°C | Homo sapiens |