Cloned (Comment) | Organism |
---|---|
expressed in HEK-293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K126A | the mutation dramatically (11fold) reduces supersulfated low molecular weight heparin affinity | Homo sapiens |
K132A | the mutation dramatically (20fold) reduces supersulfated low molecular weight heparin affinity | Homo sapiens |
N129A | the mutation moderately (5fold) reduces supersulfated low molecular weight heparin affinity | Homo sapiens |
N178A | the mutant shows wild type affinity towards supersulfated low molecular weight heparin | Homo sapiens |
R165A | the mutation moderately (5fold) reduces supersulfated low molecular weight heparin affinity | Homo sapiens |
R170A | the mutation slightly (0.5fold) reduces supersulfated low molecular weight heparin affinity | Homo sapiens |
R233A | the mutation dramatically (14fold) reduces supersulfated low molecular weight heparin affinity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
supersulfated low molecular weight heparin | inhibits the intrinsic tenase (factor IXa-factor VIIIa) complex in an antithrombin-independent manner by 90% when using factor X as substrate. Supersulfated low molecular weight heparin is 32fold more potent than unmodified low molecular weight heparin in the inhibition of factor X activation by the intrinsic tenase complex | Homo sapiens | |
unmodified low molecular weight heparin | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Factor X + H2O | Homo sapiens | - |
Factor Xa + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Factor X + H2O | - |
Homo sapiens | Factor Xa + ? | - |
? | |
Nalpha-benzyloxycarbonyl-D-Arg-Gly-Arg-4-nitroanilide + H2O | S-2765 | Homo sapiens | Nalpha-benzyloxycarbonyl-D-Arg-Gly-Arg + 4-nitroaniline | - |
? | |
Pefachrome IXa + H2O | i.e. CH3O2-D-CHG-Gly-Arg-4-nitroanilide | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
factor IXa | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000025 | - |
supersulfated low molecular weight heparin | mutant enzyme R170A, using factor X as substrate, at pH 7.4, 22°C | Homo sapiens | |
0.000003 | - |
supersulfated low molecular weight heparin | wild type enzyme, using factor X as substrate, at pH 7.4, 22°C | Homo sapiens | |
0.0000039 | - |
supersulfated low molecular weight heparin | mutant enzyme N178A, using factor X as substrate, at pH 7.4, 22°C | Homo sapiens | |
0.0000042 | - |
supersulfated low molecular weight heparin | mutant enzyme N129A, using factor X as substrate, at pH 7.4, 22°C | Homo sapiens | |
0.00001 | - |
supersulfated low molecular weight heparin | mutant enzyme K132A, using factor X as substrate, at pH 7.4, 22°C | Homo sapiens | |
0.00001 | - |
unmodified low molecular weight heparin | mutant enzyme N129A, using factor X as substrate, at pH 7.4, 22°C | Homo sapiens | |
0.000012 | - |
unmodified low molecular weight heparin | mutant enzyme R170A, using factor X as substrate, at pH 7.4, 22°C | Homo sapiens | |
0.000012 | - |
unmodified low molecular weight heparin | wild type enzyme, using factor X as substrate, at pH 7.4, 22°C | Homo sapiens | |
0.0000141 | - |
unmodified low molecular weight heparin | mutant enzyme N178A, using factor X as substrate, at pH 7.4, 22°C | Homo sapiens | |
0.000017 | - |
supersulfated low molecular weight heparin | mutant enzyme R165A, using factor X as substrate, at pH 7.4, 22°C | Homo sapiens | |
0.000049 | - |
unmodified low molecular weight heparin | mutant enzyme K132A, using factor X as substrate, at pH 7.4, 22°C | Homo sapiens | |
0.000072 | - |
supersulfated low molecular weight heparin | mutant enzyme K126A, using factor X as substrate, at pH 7.4, 22°C | Homo sapiens | |
0.00016 | - |
unmodified low molecular weight heparin | mutant enzyme R165A, using factor X as substrate, at pH 7.4, 22°C | Homo sapiens | |
0.00017 | - |
supersulfated low molecular weight heparin | mutant enzyme R233A, using factor X as substrate, at pH 7.4, 22°C | Homo sapiens | |
0.00032 | - |
unmodified low molecular weight heparin | mutant enzyme K126A, using factor X as substrate, at pH 7.4, 22°C | Homo sapiens | |
0.00147 | - |
unmodified low molecular weight heparin | mutant enzyme R233A, using factor X as substrate, at pH 7.4, 22°C | Homo sapiens |