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Literature summary for 3.4.21.22 extracted from

  • Neuenschwander, P.F.; Williamson, S.R.; Nalian, A.; Baker-Deadmond, K.J.
    Heparin modulates the 99-loop of factor IXa: effects on reactivity with isolated Kunitz-type inhibitor domains (2006), J. Biol. Chem., 281, 23066-23074.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
anionic phospholipid highly activating Homo sapiens
vitamin K dependent on Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in HEK-293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
K98A site-directed mutagenesis, Homo sapiens
additional information the modulatory effect of heparin can be completely abrogated by mutating a single amino acid residue in the 99-loop region of the extended fIXa active site cleft outside of the heparin binding exosite, overview Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
antithrombin in vivo and in vitro inhibition, the interaction is improved by heparin binding via the formation of bridged complexes where heparin acts as a template Homo sapiens
BPTI Kunitz-type inhibitor, competitive, reversible inhibition, low molecular weight heparin binding to fIXa enhances reactivity of fIXa with the Kunitz-type inhibitor BPTI Homo sapiens
enoxaparin competitive, reversible inhibition Homo sapiens
additional information oligosaccharide binding at loop 99 can allosterically modulate the fIXa active site region, overview Homo sapiens
PN2-KPI Kunitz-type inhibitor, competitive, reversible inhibition Homo sapiens
TFPI-K1 Kunitz-type inhibitor, competitive, reversible inhibition Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ hughly activating Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
factor X + H2O Homo sapiens factor IXa is a vitamin K-dependent blood coagulation factor that is essential for the amplification or consolidation phase of blood coagulation activated factor X + ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P00740
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification activation by purified factor X activator from the venom of Russell┬ĺs Vipe cleaving fIX after Arg180 to generate active enzyme fIXaalpha Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from HEK-293 cells by anion exchange and heparin affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation from plasma Homo sapiens
-
plasma
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CH3SO2-D-Leu-Gly-Arg-4-nitroanilide + H2O commercial chromogenic substrate CBS 31.39 Homo sapiens ?
-
?
factor X + H2O factor IXa is a vitamin K-dependent blood coagulation factor that is essential for the amplification or consolidation phase of blood coagulation Homo sapiens activated factor X + ?
-
?
factor X + H2O oligosaccharide binding at loop 99 can allosterically modulate the fIXa active site region Homo sapiens activated factor X + ?
-
?

Synonyms

Synonyms Comment Organism
factor IXa
-
Homo sapiens
FIXa
-
Homo sapiens
More the enzyme is a member of the serine protease family and shares a high degree of homology with trypsin Homo sapiens

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics Homo sapiens