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Literature summary for 3.4.21.22 extracted from

  • Yang, X.; Chang, Y.J.; Lin, S.W.; Walsh, P.N.
    Identification of residues Asn89, Ile90, and Val107 of the factor IXa second epidermal growth factor domain that are essential for the assembly of the factor X-activating complex on activated platelets (2004), J. Biol. Chem., 279, 46400-46405.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
rFIXa104A recombinant FIXa alanine point mutation in loop 2 of the EGF2 domain Homo sapiens
rFIXa105A recombinant FIXa alanine point mutation in loop 2 of the EGF2 domain Homo sapiens
rFIXa107A recombinant FIXa alanine point mutation in loop 2 of the EGF2 domain Homo sapiens
rFIXa89A recombinant FIXa alanine point mutation in loop 1 of the EGF2 domain Homo sapiens
rFIXa90A recombinant FIXa alanine point mutation in loop 1 of the EGF2 domain Homo sapiens
rFIXa91A recombinant FIXa alanine point mutation in loop 1 of the EGF2 domain Homo sapiens
rFIXa94A recombinant FIXa alanine point mutation in loop 1 of the EGF2 domain Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000068
-
Factor X mutant enzyme rFIXa90A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 ┬░C Homo sapiens
0.0000089
-
Factor X mutant enzyme rFIXa107A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 ┬░C Homo sapiens
0.0000181
-
Factor X mutant enzyme rFIXa90A, 5 units/ml FVIIIa and extruded phosphatidylcholine-phosphatidylserine phospholipid vesicles supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 ┬░C Homo sapiens
0.00002
-
Factor X mutant enzyme rFIXa104A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 ┬░C Homo sapiens
0.0000224
-
Factor X mutant enzyme rFIXa94A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 ┬░C Homo sapiens
0.0000251
-
Factor X mutant enzyme rFIXa105A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 ┬░C Homo sapiens
0.0000295
-
Factor X mutant enzyme rFIXa91A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 ┬░C Homo sapiens
0.000034
-
Factor X mutant enzyme rFIXa107A, 5 units/ml FVIIIa and extruded phosphatidylcholine-phosphatidylserine phospholipid vesicles supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 ┬░C Homo sapiens
0.0000396
-
Factor X plasma-derived FIXa, 5 units/ml FVIIIa and extruded phosphatidylcholine-phosphatidylserine phospholipid vesicles supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 ┬░C Homo sapiens
0.0000428
-
Factor X plasma-derived FIXa, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 ┬░C Homo sapiens
0.0000629
-
Factor X mutant enzyme rFIXa91A, 5 units/ml FVIIIa and extruded phosphatidylcholine-phosphatidylserine phospholipid vesicles supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 ┬░C Homo sapiens
0.000064
-
Factor X mutant enzyme rFIXa94A, 5 units/ml FVIIIa and extruded phosphatidylcholine-phosphatidylserine phospholipid vesicles supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 ┬░C Homo sapiens
0.0000787
-
Factor X mutant enzyme rFIXa104A, 5 units/ml FVIIIa and extruded phosphatidylcholine-phosphatidylserine phospholipid vesicles supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 ┬░C Homo sapiens
0.0000869
-
Factor X mutant enzyme rFIXa105A, 5 units/ml FVIIIa and extruded phosphatidylcholine-phosphatidylserine phospholipid vesicles supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 ┬░C Homo sapiens
0.0001542
-
Factor X mutant enzyme rFIXa89A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 ┬░C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
factor X + H2O Homo sapiens in the presence of FVIIIa (5 units/ml) at 37 ┬░C factor Xa
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P00740
-
-

Purification (Commentary)

Purification (Comment) Organism
ion exchange chromatography on Q-Sepharose Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
factor X + H2O
-
Homo sapiens factor Xa
-
?
factor X + H2O in the presence of FVIIIa (5 units/ml) at 37 ┬░C Homo sapiens factor Xa
-
?
N-alpha-benzyloxycarbonyl-D-arginylglycyl-L-arginine-para-nitroanilide + H2O
-
Homo sapiens N-alpha-benzyloxycarbonyl-D-arginylglycyl-L-arginine + para-nitroaniline
-
?

Synonyms

Synonyms Comment Organism
factor IXa
-
Homo sapiens
FIXa
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.9
-
Factor X mutant enzyme rFIXa104A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 ┬░C Homo sapiens
2.9
-
Factor X mutant enzyme rFIXa94A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 ┬░C Homo sapiens
3.3
-
Factor X mutant enzyme rFIXa107A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 ┬░C Homo sapiens
3.4
-
Factor X plasma-derived FIXa, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 ┬░C Homo sapiens
3.8
-
Factor X mutant enzyme rFIXa105A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 ┬░C Homo sapiens
4.1
-
Factor X mutant enzyme rFIXa90A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 ┬░C Homo sapiens
5.2
-
Factor X mutant enzyme rFIXa91A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 ┬░C Homo sapiens
16.6
-
Factor X mutant enzyme rFIXa89A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 ┬░C Homo sapiens