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Literature summary for 3.4.21.21 extracted from

  • Sorensen, A.B.; Madsen, J.J.; Svensson, L.A.; Pedersen, A.A.; Ostergaard, H.; Overgaard, M.T.; Olsen, O.H.; Gandhi, P.S.
    Molecular basis of enhanced activity in factor VIIa-trypsin variants conveys insights into tissue factor-mediated allosteric regulation of factor VIIa activity (2016), J. Biol. Chem., 291, 4671-4683 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.21

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M sodium citrate buffer at pH 5.1, 16.6% PEG 3350, 12.5% (v/v) 1-propanol, or 0.1 M cacodylate buffer at pH 5.6, 12% (w/v) PEG 8000 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4-aminobenzamidine
-
 Homo sapiens
D-Phe-Phe-Arg chloromethylketone
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
 D-Ile-Pro-Arg-4-nitroanilide wild type enzyme, in the presence of soluble tissue factor (1-219), at pH 7.4 and 25°C Homo sapiens
8.9
-
 D-Ile-Pro-Arg-4-nitroanilide wild type enzyme, in the absence of soluble tissue factor (1-219), at pH 7.4 and 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P08709
-
-

Purification (Commentary)

Purification (Comment) Organism
ResourceQ column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ile-Pro-Arg-4-nitroanilide + H2O S-2288 Homo sapiens D-Ile-Pro-Arg + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
FVIIa
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.7
-
 D-Ile-Pro-Arg-4-nitroanilide wild type enzyme, in the absence of soluble tissue factor (1-219), at pH 7.4 and 25°C Homo sapiens
34.6
-
 D-Ile-Pro-Arg-4-nitroanilide wild type enzyme, in the presence of soluble tissue factor (1-219), at pH 7.4 and 25°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0493
-
 4-aminobenzamidine wild type enzyme, in the presence of soluble tissue factor (1-219), at pH 7.4 and 25°C Homo sapiens
1.485
-
 4-aminobenzamidine wild type enzyme, in the absence of soluble tissue factor (1-219), at pH 7.4 and 25°C Homo sapiens

General Information

General Information Comment Organism
physiological function the complex of coagulation factor VIIa and membrane-bound tissue factor initiates blood coagulation upon vascular injury Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.9
-
 D-Ile-Pro-Arg-4-nitroanilide wild type enzyme, in the absence of soluble tissue factor (1-219), at pH 7.4 and 25°C Homo sapiens
28
-
 D-Ile-Pro-Arg-4-nitroanilide wild type enzyme, in the presence of soluble tissue factor (1-219), at pH 7.4 and 25°C Homo sapiens