Cloned (Comment) | Organism |
---|---|
expressed in baby hamster kidney cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C164V/V299C-FVIIa | introduction of a new disulfide bridge between Cys-159 and an introduced Cys at position 299 | Homo sapiens |
L280I/V299M-FVIIa | without description | Homo sapiens |
V299M-FVIIa | modification of the first Leu-X-Val motif by the introduction of Met in the third position | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide | wild type enzyme, in the presence of tissue factor, pH 7.4 | Homo sapiens | |
2 | - |
H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide | mutant enzyme L280I/V299M-FVIIa, in the presence of tissue factor, pH 7.4 | Homo sapiens | |
2.2 | - |
H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide | mutant enzyme C164V/V299C FVIIa, in the presence of tissue factor, pH 7.4 | Homo sapiens | |
2.3 | - |
H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide | mutant enzyme V299M-FVIIa, in the presence of tissue factor, pH 7.4 | Homo sapiens | |
6.6 | - |
H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide | mutant enzyme L280I/V299M-FVIIa, pH 7.4 | Homo sapiens | |
8 | - |
H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide | wild type enzyme, pH 7.4 | Homo sapiens | |
9.2 | - |
H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide | mutant enzyme C164V/V299C, pH 7.4 | Homo sapiens | |
9.8 | - |
H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide | mutant enzyme V299M-FVIIa, pH 7.4 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
method not mentioned | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
factor X + H2O | - |
Homo sapiens | factor Xa | - |
? | |
H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide + H2O | i.e. S-2288 | Homo sapiens | H-D-isoleucyl-L-prolyl-arginine + p-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
factor VIIa | - |
Homo sapiens |