Activating Compound | Comment | Organism | Structure |
---|---|---|---|
tissue factor | activates | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in CHO-K1 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
DELTA360-329 | lower affinity for soluble tissue factor as compared to wild-type factor VIIa, 7fold smaller tissue factor-mediated acceleration of amidolytic activity compared to wild type factor VIIa | Homo sapiens |
additional information | mutant enzyme which has all of the residues of the loop formed by a disulfide bond between Cys310 and Cys329 replaced with those of trypsin has lower affinity for soluble tissue factor as compared to wild-type enzyme, 2fold smaller tissue factor-mediated acceleration of amidolytic activity compared to wild type enzyme, the catalytic efficiencies of the mutant towards various chromogenic substrates are 2-18fold greater than those of the wild-type factor VIIa. As well as the wild-type enzyme the mutant exists predominantly in the zymogen-like state | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.25 | - |
D-Ile-Pro-Arg-p-nitroanilide | pH 8.0, 30°C, plasma derived wild-type enzyme, activated by tissue factor | Homo sapiens | |
1.43 | - |
D-Ile-Pro-Arg-p-nitroanilide | pH 8.0, 30°C, activated wild-type enzyme, activated by tissue factor | Homo sapiens | |
1.53 | - |
D-Ile-Pro-Arg-p-nitroanilide | pH 8.0, 30°C, mutant enzyme DELTA360-329, without tissue factor | Homo sapiens | |
1.73 | - |
D-Ile-Pro-Arg-p-nitroanilide | pH 8.0, 30°C, mutant enzyme which has all of the residues of the loop formed by a disulfide bond between Cys310 and Cys329 replaced with those of trypsin, activated by tissue factor | Homo sapiens | |
2.22 | - |
D-Ile-Pro-Arg-p-nitroanilide | pH 8.0, 30°C, activated wild-type enzyme, without tissue factor | Homo sapiens | |
2.82 | - |
D-Ile-Pro-Arg-p-nitroanilide | pH 8.0, 30°C, plasma derived wild-type enzyme, without tissue factor | Homo sapiens | |
2.98 | - |
D-Ile-Pro-Arg-p-nitroanilide | pH 8.0, 30°C, mutant enzyme DELTA360-329, activated by tissue factor | Homo sapiens | |
3.06 | - |
D-Ile-Pro-Arg-p-nitroanilide | pH 8.0, 30°C, mutant enzyme which has all of the residues of the loop formed by a disulfide bond between Cys310 and Cys329 replaced with those of trypsin, without tissue factor | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes expressed in CHO-K1 cells | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood plasma | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-Ile-Glu-gamma-methoxyglutamyl-Gly-Arg-p-nitroanilide + H2O | i.e. S2222, 2% of the activity with N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide, plasma-derived wild-type enzyme | Homo sapiens | benzoyl-Ile-Glu-gamma-methoxyglutamyl-Gly-Arg + p-nitroaniline | - |
? | |
benzyloxycarbonyl-D-Arg-Gly-Arg + H2O | i.e. S2765, 6% of the activity with N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide, plasma-derived wild-type enzyme | Homo sapiens | ? | - |
? | |
D-Ile-Pro-Arg-p-nitroanilide + H2O | i.e. S2288, 68% of the activity with N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide, plasma-derived wild-type enzyme | Homo sapiens | D-Ile-Pro-Arg + p-nitroaniline | - |
? | |
D-Phe-pipecolyl-Arg-p-nitroanilide + H2O | i.e. S2238, 34% of the activity with N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide, plasma-derived wild-type enzyme | Homo sapiens | D-Phe-pipecolyl-Arg + p-nitroaniline | - |
? | |
D-Pro-Phe-Arg-p-nitroanilide + H2O | i.e. S2302, 12% of the activity with N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide, plasma-derived wild-type enzyme | Homo sapiens | D-Pro-Phe-Arg + p-nitroaniline | - |
? | |
N-methoxycarbonyl-D-Nle-Gly-Arg-p-nitroanilide + H2O | i.e. chromozym X, 11% of the activity with N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide, plasma-derived wild-type enzyme | Homo sapiens | N-methoxycarbonyl-D-Nle-Gly-Arg + p-nitroaniline | - |
? | |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide + H2O | i.e. chromozym t-PA | Homo sapiens | N-methylsulfonyl-D-Phe-Gly-Arg + p-nitroaniline | - |
? | |
pyroglutamyl-Gly-Arg-p-nitroanilide + H2O | i.e. S2444, 2% of the activity with N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide, plasma-derived wild-type enzyme | Homo sapiens | pyroglutamyl-Gly-Arg + p-nitroaniline | - |
? | |
pyroglutamyl-Phe-Lys-p-nitroanilide + H2O | i.e. S2403, 1.4% of the activity with N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide, plasma-derived wild-type enzyme | Homo sapiens | pyroglutamyl-D-Phe-Gly-Arg + p-nitroaniline | - |
? | |
pyroglutamyl-Pro-Arg-p-nitroanilide + H2O | i.e. S2366, 38% of the activity with N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide, plasma-derived wild-type enzyme | Homo sapiens | pyroglutamyl-Pro-Arg + p-nitroaniline | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.59 | - |
D-Ile-Pro-Arg-p-nitroanilide | pH 8.0, 30°C, mutant enzyme DELTA360-329, without tissue factor | Homo sapiens | |
1.95 | - |
D-Ile-Pro-Arg-p-nitroanilide | pH 8.0, 30°C, activated wild-type enzyme, without tissue factor | Homo sapiens | |
3.29 | - |
D-Ile-Pro-Arg-p-nitroanilide | pH 8.0, 30°C, plasma derived wild-type enzyme, without tissue factor | Homo sapiens | |
7.81 | - |
D-Ile-Pro-Arg-p-nitroanilide | pH 8.0, 30°C, mutant enzyme DELTA360-329, activated by tissue factor | Homo sapiens | |
18.53 | - |
D-Ile-Pro-Arg-p-nitroanilide | pH 8.0, 30°C, mutant enzyme which has all of the residues of the loop formed by a disulfide bond between Cys310 and Cys329 replaced with those of trypsin, without tissue factor | Homo sapiens | |
20.98 | - |
D-Ile-Pro-Arg-p-nitroanilide | pH 8.0, 30°C, mutant enzyme which has all of the residues of the loop formed by a disulfide bond between Cys310 and Cys329 replaced with those of trypsin, activated by tissue factor | Homo sapiens | |
37.08 | - |
D-Ile-Pro-Arg-p-nitroanilide | pH 8.0, 30°C, activated wild-type enzyme, activated by tissue factor | Homo sapiens | |
44.2 | - |
D-Ile-Pro-Arg-p-nitroanilide | pH 8.0, 30°C, plasma derived wild-type enzyme, activated by tissue factor | Homo sapiens |