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Literature summary for 3.4.21.21 extracted from

  • Neuenschwander, P.F.; Vernon, J.T.; Morrissey, J.H.
    Tissue factor alters the pKa values of catalytically important factor VIIa residues (2002), Biochemistry, 41, 3364-3371.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
tissue factor tissue factor binding to factor VIIa allosterically augments the enzymatic activity of factor VIIa towards macromolecular substrates and small peptidyl substrates. Tissue factor binding to factor VIIa alters the chemical environment of the active site of factor VIIa by protecting Ile153 from deprotonation in the free enzyme while deprotecting the catalytic triad as a whole when in the Michaelis complex Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 6.5, MTE buffer, without tissue factor Homo sapiens
2.3
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 6.5, MES buffer, without tissue factor Homo sapiens
6.3
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 7.5, Tris buffer, without tissue factor Homo sapiens
6.4
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 9.5, BTP buffer, without tissue factor Homo sapiens
6.8
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 9.5, ethanolamine buffer, without tissue factor Homo sapiens
8
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 7.5, HEPES buffer, without tissue factor Homo sapiens
10
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 7.5, MTE buffer, without tissue factor Homo sapiens
13
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 9.5, MTE buffer, without tissue factor Homo sapiens
50
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 9.5, veronal buffer, without tissue factor Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide + H2O i.e. chromozym t-PA Homo sapiens N-methylsulfonyl-D-Phe-Gly-Arg + p-nitroaniline
-
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Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1500
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 6.5, MTE buffer, without tissue factor Homo sapiens
1560
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 6.5, MES buffer, without tissue factor Homo sapiens
5940
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 9.5, BTP buffer, without tissue factor Homo sapiens
7200
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 9.5, ethanolamine buffer, without tissue factor Homo sapiens
9600
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 9.5, MTE buffer, without tissue factor Homo sapiens
31800
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 7.5, Tris buffer, without tissue factor Homo sapiens
33600
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 7.5, HEPES buffer, without tissue factor Homo sapiens
38400
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 7.5, MTE buffer, without tissue factor Homo sapiens
84000
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 9.5, veronal buffer, without tissue factor Homo sapiens
96000
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 6.5, MES buffer, in presence of tissue factor Homo sapiens
96000
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 6.5, MTE buffer, in presence of tissue factor Homo sapiens
198000
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 7.5, HEPES buffer, in presence of tissue factor Homo sapiens
216000
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 7.5, MTE buffer, in presence of tissue factor Homo sapiens
246000
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 7.5, Tris buffer, in presence of tissue factor Homo sapiens
324000
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 9.5, veronal buffer, in presence of tissue factor Homo sapiens
360000
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 9.5, MTE buffer, in presence of tissue factor Homo sapiens
366000
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 9.5, BTP buffer, in presence of tissue factor Homo sapiens
396000
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 9.5, ethanolamine buffer, in presence of tissue factor Homo sapiens