Activating Compound | Comment | Organism | Structure |
---|---|---|---|
tissue factor | tissue factor binding to factor VIIa allosterically augments the enzymatic activity of factor VIIa towards macromolecular substrates and small peptidyl substrates. Tissue factor binding to factor VIIa alters the chemical environment of the active site of factor VIIa by protecting Ile153 from deprotonation in the free enzyme while deprotecting the catalytic triad as a whole when in the Michaelis complex | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 6.5, MTE buffer, without tissue factor | Homo sapiens | |
2.3 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 6.5, MES buffer, without tissue factor | Homo sapiens | |
6.3 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 7.5, Tris buffer, without tissue factor | Homo sapiens | |
6.4 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 9.5, BTP buffer, without tissue factor | Homo sapiens | |
6.8 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 9.5, ethanolamine buffer, without tissue factor | Homo sapiens | |
8 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 7.5, HEPES buffer, without tissue factor | Homo sapiens | |
10 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 7.5, MTE buffer, without tissue factor | Homo sapiens | |
13 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 9.5, MTE buffer, without tissue factor | Homo sapiens | |
50 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 9.5, veronal buffer, without tissue factor | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide + H2O | i.e. chromozym t-PA | Homo sapiens | N-methylsulfonyl-D-Phe-Gly-Arg + p-nitroaniline | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1500 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 6.5, MTE buffer, without tissue factor | Homo sapiens | |
1560 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 6.5, MES buffer, without tissue factor | Homo sapiens | |
5940 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 9.5, BTP buffer, without tissue factor | Homo sapiens | |
7200 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 9.5, ethanolamine buffer, without tissue factor | Homo sapiens | |
9600 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 9.5, MTE buffer, without tissue factor | Homo sapiens | |
31800 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 7.5, Tris buffer, without tissue factor | Homo sapiens | |
33600 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 7.5, HEPES buffer, without tissue factor | Homo sapiens | |
38400 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 7.5, MTE buffer, without tissue factor | Homo sapiens | |
84000 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 9.5, veronal buffer, without tissue factor | Homo sapiens | |
96000 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 6.5, MES buffer, in presence of tissue factor | Homo sapiens | |
96000 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 6.5, MTE buffer, in presence of tissue factor | Homo sapiens | |
198000 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 7.5, HEPES buffer, in presence of tissue factor | Homo sapiens | |
216000 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 7.5, MTE buffer, in presence of tissue factor | Homo sapiens | |
246000 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 7.5, Tris buffer, in presence of tissue factor | Homo sapiens | |
324000 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 9.5, veronal buffer, in presence of tissue factor | Homo sapiens | |
360000 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 9.5, MTE buffer, in presence of tissue factor | Homo sapiens | |
366000 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 9.5, BTP buffer, in presence of tissue factor | Homo sapiens | |
396000 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 9.5, ethanolamine buffer, in presence of tissue factor | Homo sapiens |