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Literature summary for 3.4.21.21 extracted from

  • Jin, J.; Chang, J.; Stafford, D.W.; Straight, D.L.
    Residues Y179 and H101 of a hydrophobic patch of factor VII are involved in activation by factor Xa (2001), Biochemistry, 40, 11405-11410.
    View publication on PubMed
Search for more literature for 3.4.21.21

Activating Compound

Activating Compound Comment Organism Structure
factor Xa activates Homo sapiens

Protein Variants

Protein Variants Comment Organism
D102Q inactive mutant enzyme Homo sapiens
D186A turnover-number for activation of factor X is 5.5fold lower than that of the wild-type factor VII, Km-value for activation of factor X is 1.2fold lower than that of the wild-type factor VII Homo sapiens
F256A turnover-number and Km-value for activation of factor X are nearly identical to that of the wild-type factor VII Homo sapiens
H101A turnover-number for activation of factor X is 1.12fold lower than that of the wild-type factor VII, Km-value for activation of factor X is 1.23fold lower than that of the wild-type factor VII. Activation by factor Xa is significantly more slowly than that of wild-type enzyme. Tissue factor affinity and small substrate activity is similar to wild-type enzyme Homo sapiens
L144A turnover-number for activation of factor X is 40fold lower than that of the wild-type factor VII, Km-value for activation of factor X is 1.6fold lower than that of the wild-type factor VII Homo sapiens
L144A/R147A/D186A turnover-number for activation of factor X is 239fold lower than that of the wild-type factor VII, Km-value for activation of factor X is 1.2fold higher than that of the wild-type factor VII Homo sapiens
N100A turnover-number for activation of factor X is 3fold lower than that of the wild-type factor VII, Km-value for activation of factor X is 1.4fold lower than that of the wild-type factor VII Homo sapiens
N100A/H101A/Y179A/F256A turnover-number for activation of factor X is 3fold lower than that of the wild-type factor VII, Km-value for activation of factor X is nearly identical to that of the wild-type factor VII. Activation by factor Xa is significantly more slowly than that of wild-type enzyme Homo sapiens
R147A turnover-number for activation of factor X is 3.7fold lower than that of the wild-type factor VII, Km-value for activation of factor X is 1.2fold lower than that of the wild-type factor VII Homo sapiens
Y179A turnover-number for activation of factor X is nearly identical to that of the wild-type factor VII, Km-value for activation of factor X is 1.6fold lower than that of the wild-type factor VII. Activation by factor Xa is significantly more slowly than that of wild-type enzyme. Tissue factor affinity and small substrate activity is similar to wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 Factor X 37°C, mutant enzyme L144A Homo sapiens
0.15
-
 Factor X 37°C, mutant enzyme N100A Homo sapiens
0.17
-
 Factor X 37°C, mutant enzyme H101A Homo sapiens
0.18
-
 Factor X 37°C, mutant enzyme D186A Homo sapiens
0.18
-
 Factor X 37°C, mutant enzyme R147A Homo sapiens
0.21
-
 Factor X 37°C, wild-type enzyme Homo sapiens
0.22
-
 Factor X 37°C, mutant enzyme F256A Homo sapiens
0.22
-
 Factor X 37°C, mutant enzyme N100A/H101A/Y179A/F256A Homo sapiens
0.23
-
 Factor X 37°C, mutant enzyme Y179A Homo sapiens
0.25
-
 Factor X 37°C, mutant enzyme L144A/R147A/D186A Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
factor X + H2O
-
 Homo sapiens activated factor X + ? i.e. factor Xa ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 Factor X 37°C, mutant enzyme L144A/R147A/D186A Homo sapiens
0.18
-
 Factor X 37°C, mutant enzyme L144A Homo sapiens
1.31
-
 Factor X 37°C, mutant enzyme D186A Homo sapiens
1.94
-
 Factor X 37°C, mutant enzyme R147A Homo sapiens
2.31
-
 Factor X 37°C, mutant enzyme N100A Homo sapiens
2.35
-
 Factor X 37°C, mutant enzyme N100A/H101A/Y179A/F256A Homo sapiens
6.32
-
 Factor X 37°C, mutant enzyme F256A Homo sapiens
6.36
-
 Factor X 37°C, mutant enzyme H101A Homo sapiens
6.91
-
 Factor X 37°C, mutant enzyme Y179A Homo sapiens
7.16
-
 Factor X 37°C, wild-type enzyme Homo sapiens