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Literature summary for 3.4.21.20 extracted from

  • Thorpe, M.; Fu, Z.; Chahal, G.; Akula, S.; Kervinen, J.; de Garavilla, L.; Hellman, L.
    Extended cleavage specificity of human neutrophil cathepsin G A low activity protease with dual chymase and tryptase-type specificities (2018), PLoS ONE, 13, e0195077 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.20

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
fibronectin + H2O Homo sapiens
-
 fragments of fibronectin
-
 ?
additional information Homo sapiens The enzyme has a dual specificity consisting of chymase and tryptase-type activities. Phe, Tyr, Trp and Leu are preferred in the P1 position. The enzyme has a preference for negatively charged amino acids in the P2A position of substrates and a preference for aliphatic amino acids both upstream and downstream of the cleavage site ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
neutrophil
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fibronectin + H2O
-
 Homo sapiens fragments of fibronectin
-
 ?
additional information The enzyme has a dual specificity consisting of chymase and tryptase-type activities. Phe, Tyr, Trp and Leu are preferred in the P1 position. The enzyme has a preference for negatively charged amino acids in the P2A position of substrates and a preference for aliphatic amino acids both upstream and downstream of the cleavage site Homo sapiens ?
-
 ?
additional information no activity with succinyl-AAPI-4-nitroanilide, succinyl-AAPA-4-nitroanilide, succinyl-AAPV-4-nitroanilide, succinyl-VLGR-4-nitroanilide, Z-GPR-4-nitroanilide, acetyl-YVAD-4-nitroanilide, acetyl-VEID-4-nitroanilide, and acetyl-IEPD-4-nitroanilide Homo sapiens ?
-
 ?
succinyl-AAPF-4-nitroanilide + H2O
-
 Homo sapiens succinyl-AAPF + 4-nitroaniline
-
 ?
succinyl-AAPL-4-nitroanilide + H2O
-
 Homo sapiens succinyl-AAPL + 4-nitroaniline
-
 ?
succinyl-LLVY-4-nitroanilide + H2O
-
 Homo sapiens succinyl-LLVY + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 28000, SDS-PAGE Homo sapiens