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Literature summary for 3.4.21.19 extracted from

  • Kakudo, S.; Kikuchi, N.; Kitadokoro, K.; Fujiwaera, T.; Nakamura, E.; Okamoto, H.; Shin, M.; Tamaki, M.; Teraoka, H.; Tsuzuki, H.; Yoshida, N.
    Purification, characterization, cloning, and expression of a glutamic acid-specific protease from Bacillus licheniformis ATCC 14580 (1992), J. Biol. Chem., 267, 23782-23788.
    View publication on PubMed
Search for more literature for 3.4.21.19

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bacillus licheniformis

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Bacillus licheniformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 benzyloxycarbonyl-Leu-Leu-Glu-p-nitroanilide
-
 Staphylococcus aureus
1.2
-
 benzyloxycarbonyl-Phe-Leu-Glu-p-nitroanilide
-
 Staphylococcus aureus
1.5
-
 benzyloxycarbonyl-Ala-Leu-Glu-p-nitroanilide
-
 Bacillus licheniformis
1.8
-
 benzyloxycarbonyl-Glu-p-nitroanilide
-
 Bacillus licheniformis
2.8
-
 benzyloxycarbonyl-Leu-Leu-Glu-p-nitroanilide
-
 Bacillus licheniformis
3.5
-
 benzyloxycarbonyl-Ala-Ala-Glu-4-nitroanilide
-
 Bacillus licheniformis
3.9
-
 benzyloxycarbonyl-Ala-Leu-Glu-p-nitroanilide
-
 Staphylococcus aureus
3.9
-
 benzyloxycarbonyl-Phe-Leu-Glu-p-nitroanilide
-
 Bacillus licheniformis
4.2
-
 benzyloxycarbonyl-Ala-Glu-p-nitroanilide
-
 Bacillus licheniformis
4.9
-
 t-butyloxycarbonyl-Ala-Ala-Glu-p-nitroanilide
-
 Bacillus licheniformis
5.5
-
 benzyloxycarbonyl-Leu-Glu-p-nitroanilide
-
 Bacillus licheniformis
7.6
-
 benzyloxycarbonyl-Ala-Ala-Glu-4-nitroanilide
-
 Staphylococcus aureus
11.6
-
 t-butyloxycarbonyl-Ala-Ala-Asp-p-nitroanilide
-
 Bacillus licheniformis
13.3
-
 benzyloxycarbonyl-Ala-Glu-p-nitroanilide
-
 Staphylococcus aureus
16.5
-
 benzyloxycarbonyl-Leu-Glu-p-nitroanilide
-
 Staphylococcus aureus
19.7
-
 t-butyloxycarbonyl-Ala-Ala-Glu-p-nitroanilide
-
 Staphylococcus aureus
24.5
-
 t-butyloxycarbonyl-Ala-Ala-Asp-p-nitroanilide
-
 Staphylococcus aureus
28.4
-
 benzyloxycarbonyl-Glu-p-nitroanilide
-
 Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 x * 25000, SDS-PAGE Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
 ATCC 14580
-
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus licheniformis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Staphylococcus aureus
additional information
-
-
 Bacillus licheniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-Asp-p-nitroanilide + H2O
-
 Staphylococcus aureus acetyl-Glu + p-nitroaniline
-
 ?
acetyl-Asp-p-nitroanilide + H2O
-
 Bacillus licheniformis acetyl-Glu + p-nitroaniline
-
 ?
acetyl-Glu-p-nitroanilide + H2O
-
 Staphylococcus aureus acetyl-Glu + p-nitroaniline
-
 ?
acetyl-Glu-p-nitroanilide + H2O
-
 Bacillus licheniformis acetyl-Glu + p-nitroaniline
-
 ?
benzyloxycarbonyl-Ala-Ala-Glu-4-nitroanilide + H2O
-
 Staphylococcus aureus benzyloxycarbonyl-Ala-Ala-Glu + 4-nitroaniline
-
 ?
benzyloxycarbonyl-Ala-Ala-Glu-4-nitroanilide + H2O
-
 Bacillus licheniformis benzyloxycarbonyl-Ala-Ala-Glu + 4-nitroaniline
-
 ?
benzyloxycarbonyl-Ala-Glu-p-nitroanilide + H2O
-
 Staphylococcus aureus benzyloxycarbonyl-Ala-Glu + p-nitroaniline
-
 ?
benzyloxycarbonyl-Ala-Glu-p-nitroanilide + H2O
-
 Bacillus licheniformis benzyloxycarbonyl-Ala-Glu + p-nitroaniline
-
 ?
benzyloxycarbonyl-Ala-Leu-Glu-p-nitroanilide + H2O
-
 Staphylococcus aureus benzyloxycarbonyl-Ala-Leu-Glu + p-nitroaniline
-
 ?
benzyloxycarbonyl-Ala-Leu-Glu-p-nitroanilide + H2O
-
 Bacillus licheniformis benzyloxycarbonyl-Ala-Leu-Glu + p-nitroaniline
-
 ?
benzyloxycarbonyl-Glu-p-nitroanilide + H2O
-
 Staphylococcus aureus benzyloxycarbonyl-Glu + p-nitroaniline
-
 ?
benzyloxycarbonyl-Glu-p-nitroanilide + H2O
-
 Bacillus licheniformis benzyloxycarbonyl-Glu + p-nitroaniline
-
 ?
benzyloxycarbonyl-Leu-Glu-p-nitroanilide + H2O
-
 Staphylococcus aureus benzyloxycarbonyl-Leu-Glu + p-nitroaniline
-
 ?
benzyloxycarbonyl-Leu-Glu-p-nitroanilide + H2O
-
 Bacillus licheniformis benzyloxycarbonyl-Leu-Glu + p-nitroaniline
-
 ?
benzyloxycarbonyl-Leu-Leu-Glu-p-nitroanilide + H2O
-
 Staphylococcus aureus benzyloxycarbonyl-Leu-Leu-Glu + p-nitroaniline
-
 ?
benzyloxycarbonyl-Leu-Leu-Glu-p-nitroanilide + H2O
-
 Bacillus licheniformis benzyloxycarbonyl-Leu-Leu-Glu + p-nitroaniline
-
 ?
benzyloxycarbonyl-Phe-Leu-Glu-p-nitroanilide + H2O
-
 Staphylococcus aureus benzyloxycarbonyl-Phe-Leu-Glu + p-nitroaniline
-
 ?
benzyloxycarbonyl-Phe-Leu-Glu-p-nitroanilide + H2O
-
 Bacillus licheniformis benzyloxycarbonyl-Phe-Leu-Glu + p-nitroaniline
-
 ?
human parathyroid hormone(13-34) + H2O
-
 Staphylococcus aureus peptides peptide fragments Lys1-Glu6, Arg8-Glu10 and Lys1-Glu10 are produced after 6 min ?
human parathyroid hormone(13-34) + H2O
-
 Bacillus licheniformis peptides peptide fragments Lys1-Glu7, Arg8-Glu10 and Trp11-Phe22 are produced after 6 min ?
t-butyloxycarbonyl-Ala-Ala-Asp-p-nitroanilide + H2O
-
 Staphylococcus aureus t-butyloxycarbonyl-Ala-Ala-Asp + p-nitroaniline
-
 ?
t-butyloxycarbonyl-Ala-Ala-Asp-p-nitroanilide + H2O
-
 Bacillus licheniformis t-butyloxycarbonyl-Ala-Ala-Asp + p-nitroaniline
-
 ?
t-butyloxycarbonyl-Ala-Ala-Glu-p-nitroanilide + H2O
-
 Staphylococcus aureus t-butyloxycarbonyl-Ala-Ala-Glu + p-nitroaniline
-
 ?
t-butyloxycarbonyl-Ala-Ala-Glu-p-nitroanilide + H2O
-
 Bacillus licheniformis t-butyloxycarbonyl-Ala-Ala-Glu + p-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE Bacillus licheniformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.16
-
 t-butyloxycarbonyl-Ala-Ala-Asp-p-nitroanilide
-
 Bacillus licheniformis
0.28
-
 benzyloxycarbonyl-Glu-p-nitroanilide
-
 Bacillus licheniformis
0.29
-
 benzyloxycarbonyl-Glu-p-nitroanilide
-
 Staphylococcus aureus
0.54
-
 t-butyloxycarbonyl-Ala-Ala-Asp-p-nitroanilide
-
 Staphylococcus aureus
5.6
-
 benzyloxycarbonyl-Ala-Glu-p-nitroanilide
-
 Staphylococcus aureus
5.9
-
 benzyloxycarbonyl-Ala-Glu-p-nitroanilide
-
 Bacillus licheniformis
11.9
-
 t-butyloxycarbonyl-Ala-Ala-Glu-p-nitroanilide
-
 Bacillus licheniformis
20.8
-
 benzyloxycarbonyl-Ala-Ala-Glu-4-nitroanilide
-
 Staphylococcus aureus
22.6
-
 benzyloxycarbonyl-Leu-Glu-p-nitroanilide
-
 Bacillus licheniformis
24
-
 t-butyloxycarbonyl-Ala-Ala-Glu-p-nitroanilide
-
 Staphylococcus aureus
30.2
-
 benzyloxycarbonyl-Leu-Leu-Glu-p-nitroanilide
-
 Staphylococcus aureus
31.4
-
 benzyloxycarbonyl-Leu-Glu-p-nitroanilide
-
 Staphylococcus aureus
33.2
-
 benzyloxycarbonyl-Phe-Leu-Glu-p-nitroanilide
-
 Staphylococcus aureus
40.9
-
 benzyloxycarbonyl-Phe-Leu-Glu-p-nitroanilide
-
 Bacillus licheniformis
40.9
-
 benzyloxycarbonyl-Ala-Ala-Glu-4-nitroanilide
-
 Bacillus licheniformis
50.8
-
 benzyloxycarbonyl-Leu-Leu-Glu-p-nitroanilide
-
 Bacillus licheniformis
60.9
-
 benzyloxycarbonyl-Ala-Leu-Glu-p-nitroanilide
-
 Bacillus licheniformis
131
-
 benzyloxycarbonyl-Ala-Leu-Glu-p-nitroanilide
-
 Bacillus licheniformis