Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Thermolysin | - |
Staphylococcus aureus | |
thermolysin v8 | - |
Staphylococcus saprophyticus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Staphylococcus cohnii subsp. cohnii |
expressed in Escherichia coli | Staphylococcus caprae |
Protein Variants | Comment | Organism |
---|---|---|
N190H/Y192H/S169A | the specific activity of the mutant is 4.5fold increased from that of the wild type enzyme. The mutant potently hydrolyzes LLE-7-amido-4-methylcomarin | Staphylococcus epidermidis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-(2-aminoethyl)benzenesulfonyl fluoride | - |
Staphylococcus caprae | |
4-(2-aminoethyl)benzenesulfonyl fluoride | - |
Staphylococcus cohnii subsp. cohnii | |
diisopropyl fluorophosphate | - |
Staphylococcus caprae | |
diisopropyl fluorophosphate | - |
Staphylococcus cohnii subsp. cohnii | |
additional information | tosyl-L-phenylalanine chloromethyl ketone (0.1 mg/ml), tosyl-L-lysine chloromethyl ketone (50 mg/ml), phenylmethylsulfonyl fluoride (1 mM), aprotinin (0.2 units/ml), leupeptin (0.1 mg/ml), pepstatin (0.01 mg/ml), and dithiothreitol (5 mM) do not inhibit the enzyme activity | Staphylococcus caprae | |
additional information | tosyl-L-phenylalanine chloromethyl ketone (0.1 mg/ml), tosyl-L-lysine chloromethyl ketone (50 mg/ml), phenylmethylsulfonyl fluoride (1 mM), aprotinin (0.2 units/ml), leupeptin (0.1 mg/ml), pepstatin (0.01 mg/ml), and dithiothreitol (5 mM) do not inhibit the enzyme activity | Staphylococcus cohnii subsp. cohnii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Staphylococcus aureus ATCC 25923 | - |
- |
- |
Staphylococcus caprae | - |
- |
- |
Staphylococcus caprae GTC 378 | - |
- |
- |
Staphylococcus cohnii subsp. cohnii | - |
- |
- |
Staphylococcus cohnii subsp. cohnii GTC 248 | - |
- |
- |
Staphylococcus epidermidis | - |
- |
- |
Staphylococcus epidermidis ATCC 14990 | - |
- |
- |
Staphylococcus saprophyticus | - |
- |
- |
Staphylococcus warneri | - |
- |
- |
Staphylococcus warneri JCM 2415 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Talon affinity column chromatography | Staphylococcus cohnii subsp. cohnii |
Talon affinity column chromatography | Staphylococcus caprae |
Synonyms | Comment | Organism |
---|---|---|
GluScoh | - |
Staphylococcus cohnii subsp. cohnii |
GluScpr | - |
Staphylococcus caprae |
GluSE | - |
Staphylococcus epidermidis |
GluSsap | - |
Staphylococcus saprophyticus |
GluV8 | - |
Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Staphylococcus aureus |
7.5 | - |
- |
Staphylococcus epidermidis |
7.5 | - |
- |
Staphylococcus saprophyticus |
7.5 | - |
- |
Staphylococcus warneri |
7.5 | - |
- |
Staphylococcus cohnii subsp. cohnii |
7.5 | - |
- |
Staphylococcus caprae |