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Literature summary for 3.4.21.19 extracted from

  • Ono, T.; Ohara-Nemoto, Y.; Shimoyama, Y.; Okawara, H.; Kobayakawa, T.; Baba, T.T.; Kimura, S.; Nemoto, T.K.
    Amino acid residues modulating the activities of staphylococcal glutamyl endopeptidases (2010), Biol. Chem., 391, 1221-1232.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Thermolysin
-
Staphylococcus aureus
thermolysin v8
-
Staphylococcus saprophyticus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Staphylococcus cohnii subsp. cohnii
expressed in Escherichia coli Staphylococcus caprae

Protein Variants

Protein Variants Comment Organism
N190H/Y192H/S169A the specific activity of the mutant is 4.5fold increased from that of the wild type enzyme. The mutant potently hydrolyzes LLE-7-amido-4-methylcomarin Staphylococcus epidermidis

Inhibitors

Inhibitors Comment Organism Structure
4-(2-aminoethyl)benzenesulfonyl fluoride
-
Staphylococcus caprae
4-(2-aminoethyl)benzenesulfonyl fluoride
-
Staphylococcus cohnii subsp. cohnii
diisopropyl fluorophosphate
-
Staphylococcus caprae
diisopropyl fluorophosphate
-
Staphylococcus cohnii subsp. cohnii
additional information tosyl-L-phenylalanine chloromethyl ketone (0.1 mg/ml), tosyl-L-lysine chloromethyl ketone (50 mg/ml), phenylmethylsulfonyl fluoride (1 mM), aprotinin (0.2 units/ml), leupeptin (0.1 mg/ml), pepstatin (0.01 mg/ml), and dithiothreitol (5 mM) do not inhibit the enzyme activity Staphylococcus caprae
additional information tosyl-L-phenylalanine chloromethyl ketone (0.1 mg/ml), tosyl-L-lysine chloromethyl ketone (50 mg/ml), phenylmethylsulfonyl fluoride (1 mM), aprotinin (0.2 units/ml), leupeptin (0.1 mg/ml), pepstatin (0.01 mg/ml), and dithiothreitol (5 mM) do not inhibit the enzyme activity Staphylococcus cohnii subsp. cohnii

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-
Staphylococcus aureus ATCC 25923
-
-
-
Staphylococcus caprae
-
-
-
Staphylococcus caprae GTC 378
-
-
-
Staphylococcus cohnii subsp. cohnii
-
-
-
Staphylococcus cohnii subsp. cohnii GTC 248
-
-
-
Staphylococcus epidermidis
-
-
-
Staphylococcus epidermidis ATCC 14990
-
-
-
Staphylococcus saprophyticus
-
-
-
Staphylococcus warneri
-
-
-
Staphylococcus warneri JCM 2415
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon affinity column chromatography Staphylococcus cohnii subsp. cohnii
Talon affinity column chromatography Staphylococcus caprae

Synonyms

Synonyms Comment Organism
GluScoh
-
Staphylococcus cohnii subsp. cohnii
GluScpr
-
Staphylococcus caprae
GluSE
-
Staphylococcus epidermidis
GluSsap
-
Staphylococcus saprophyticus
GluV8
-
Staphylococcus aureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Staphylococcus aureus
7.5
-
-
Staphylococcus epidermidis
7.5
-
-
Staphylococcus saprophyticus
7.5
-
-
Staphylococcus warneri
7.5
-
-
Staphylococcus cohnii subsp. cohnii
7.5
-
-
Staphylococcus caprae