Literature summary for 3.4.21.19 extracted from

Trachuk, L.A.; Shcheglov, A.S.; Milgotina, E.I.; Chestukhina, G.G.
In vitro maturation pathway of a glutamyl endopeptidase precursor from Bacillus licheniformis (2005), Biochimie, 87, 529-537.

Search for more literature for 3.4.21.19

Application

Application	Comment	Organism
synthesis	recombinant expression of enzyme as insoluble inclusion bodies, solubilization in 6 M guanidine-HCl in presence of reducing agent and renaturation by fast frequent dilution method. Highest yield of refolded protein at pH 8.4, 4°C. Renaturation is accompanied by gradual splitting of K12-E13 and T47-E48 bonds resulting in a 26 kDa protein that is converted to 25 kDa mature protein by limited proteolysis trypsin or subtilisin. Complete cleavage of N-terminal pro-peptide is necessary for final packing and activation of enzyme	Bacillus licheniformis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	recombinant expression as insoluble inclusion bodies	Bacillus licheniformis	5737	-

Organism

Organism	UniProt	Comment	Textmining
Bacillus licheniformis	-	recombinant enzyme	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	proteolytic modification of native precursor results in a 25 kDa protein. Recombinant expression of enzyme in Escherichia coli with subsequent renaturation of inclusion bodies results in a 26 kDa protein that is converted to 25 kDa mature protein by limited proteolysis trypsin or subtilisin. Clomplete cleavage of N-terminal pro-peptide is necessary for final packing and activation of enzyme	Bacillus licheniformis

Renatured (Commentary)

Renatured (Comment)	Organism
recombinant expression of enzyme as insoluble inclusion bodies, solubilization in 6 M guanidine-HCl in presence of reducing agent and renaturation by fast frequent dilution method. Highest yield of refolded protein at pH 8.4, 4°C. Renaturation is accompanied by gradual splitting of K12-E13 and T47-E48 bonds resulting in a 26 kDa protein	Bacillus licheniformis

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Release 2023.1 (January 2023)