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Literature summary for 3.4.21.19 extracted from

  • Gabdrakhmanova, L.A.; Balaban, N.P.; Sharipova, M.R.; Kostrov, S.V.; Akimkina, T.V.; Rudenskaya, G.N.; Leshchinskaya, I.B.
    Optimization of Bacillus intermedius glutamyl endopeptidase production by recombinant strain of Bacillus subtilis and localization of glutamyl endopeptidase in Bacillus subtilis cells (2002), Enzyme Microb. Technol., 31, 256-263.
No PubMed abstract available
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Bacillus subtilis, optimization of culture medium composition for production of recombinant enzyme, recombinant enzyme is secreted to the medium from Bacillus subtilis cells, 2-5% of the enzyme remain bound to the cell wall Bacillus intermedius

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ gradual inhibition of enzyme expression in recombinant Bacillus subtilis strain at 1-10 mM Bacillus intermedius
Fe2+ gradual inhibition of enzyme expression in recombinant Bacillus subtilis strain at 1-10 mM Bacillus intermedius
Zn2+ gradual inhibition of enzyme expression in recombinant Bacillus subtilis strain at 1-10 mM Bacillus intermedius

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound Bacillus intermedius 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulation of enzyme expression in recombinant Bacillus subtilis strain at 2 mM Bacillus intermedius
Co2+ strong stimulation, up to 400%, of enzyme expression in recombinant Bacillus subtilis strain at 5 mM Bacillus intermedius
Mg2+ stimulation of enzyme expression in recombinant Bacillus subtilis strain at 1 mM Bacillus intermedius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Gelatin + H2O Bacillus intermedius
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus intermedius
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gelatin + H2O
-
 Bacillus intermedius ?
-
 ?