KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of the acyl transfer reactions with substrate mimetics | Staphylococcus aureus | |
additional information | - |
additional information | kinetics of the acyl transfer reactions with substrate mimetics | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
- |
- |
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
further purification of commercial preparation | Bacillus licheniformis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Staphylococcus aureus | - |
commercial preparation | - |
Bacillus licheniformis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
hydrolysis activity of the enzyme with substrate mimetics | Staphylococcus aureus |
additional information | - |
hydrolysis activity of the enzyme with substrate mimetics | Bacillus licheniformis |
360 | - |
- |
Bacillus licheniformis |
800 | - |
- |
Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-Glu-4-nitrophenyl + H2O | - |
Staphylococcus aureus | acetyl-Glu + 4-nitrophenol | - |
? | |
acetyl-Glu-4-nitrophenyl ester + H2O | - |
Bacillus licheniformis | acetyl-Glu + 4-nitrophenol | - |
? | |
benzyloxycarbonyl-Ala 2-carboxyphenylthioester + H2O | - |
Bacillus licheniformis | ? | - |
? | |
benzyloxycarbonyl-Ala 2-carboxyphenylthioester + H2O | - |
Staphylococcus aureus | benzyloxycarbonyl-Ala + 2-carboxyphenylthiol | - |
? | |
benzyloxycarbonyl-Ala 3-carboxyphenylester + H2O | - |
Bacillus licheniformis | benzyloxycarbonyl-Ala + 3-hydroxybenzoate | - |
? | |
benzyloxycarbonyl-Ala 3-carboxyphenylester + H2O | - |
Staphylococcus aureus | benzyloxycarbonyl-Ala + 3-carboxyphenol | - |
? | |
benzyloxycarbonyl-Ala 4-carboxyphenylester + H2O | - |
Bacillus licheniformis | benzyloxycarbonyl-Ala + 4-hydroxybenzoate | - |
? | |
benzyloxycarbonyl-Ala 4-carboxyphenylester + H2O | - |
Staphylococcus aureus | benzyloxycarbonyl-Ala + 4-carboxyphenol | - |
? | |
benzyloxycarbonyl-Ala carboxyethylthioester + H2O | - |
Bacillus licheniformis | ? | - |
? | |
benzyloxycarbonyl-Ala carboxyethylthioester + H2O | - |
Staphylococcus aureus | benzyloxycarbonyl-Ala + carboxyethylthiol | - |
? | |
benzyloxycarbonyl-Ala carboxymethylthioester + H2O | - |
Bacillus licheniformis | ? | - |
? | |
benzyloxycarbonyl-Ala carboxymethylthioester + H2O | - |
Staphylococcus aureus | benzyloxycarbonyl-Ala + carboxymethylthiol | - |
? | |
benzyloxycarbonyl-Asp methyl ester + H2O | - |
Staphylococcus aureus | benzyloxycarbonyl-Asp + methanol | - |
? | |
benzyloxycarbonyl-Asp methyl ester + H2O | - |
Bacillus licheniformis | benzyloxycarbonyl-Asp + methanol | - |
? | |
benzyloxycarbonyl-Glu methyl ester + H2O | - |
Staphylococcus aureus | benzyloxycarbonyl-Glu + methanol | - |
? | |
benzyloxycarbonyl-Glu methyl ester + H2O | - |
Bacillus licheniformis | benzyloxycarbonyl-Glu + methanol | - |
? | |
benzyloxycarbonyl-Glu methylthioester + H2O | - |
Bacillus licheniformis | benzyloxycarbonyl-Glu + methylthiol | - |
? | |
benzyloxycarbonyl-Glu methylthioester + H2O | - |
Staphylococcus aureus | benzyloxycarbonyl-L-Glu + methylthiol | - |
? | |
additional information | enzyme also performs acyl-transfer reactions with substrate mimetics carboxymethyl acylthioester, carboxyethyl acylthioester, 2-carboxyphenyl acylthioester, 3-carboxyphenyl acylester, 4-carboxyphenyl acylester | Staphylococcus aureus | ? | - |
? | |
additional information | enzyme also performs acyl-transfer reactions with substrate mimetics carboxymethyl acylthioester, carboxyethyl acylthioester, 2-carboxyphenyl acylthioester, 3-carboxyphenyl acylester, 4-carboxyphenyl acylester | Bacillus licheniformis | ? | - |
? | |
N-tert-butyloxycarbonyl-L-Glu-alpha-phenyl ester + H2O | - |
Staphylococcus aureus | butyloxycarbonyl-L-Glu + phenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BL-GSE | - |
Bacillus licheniformis |
Glu-specific endopeptidase | - |
Bacillus licheniformis |
V8 protease | - |
Staphylococcus aureus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Staphylococcus aureus |
37 | - |
assay at | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bacillus licheniformis |
7.8 | - |
assay at | Staphylococcus aureus |