Literature summary for 3.4.21.120 extracted from

Hardy, D.M.; Hedrick, J.L.
Oviductin. Purification and properties of the oviductal protease that processes the molecular weight 43000 glycoprotein of the Xenopus laevis egg envelope (1992), Biochemistry, 31, 4466-4472.

Search for more literature for 3.4.21.120

General Stability

General Stability	Organism
relatively stable to rapid freezing in the presence of 20% glycerol. 90% of the activity remains after each freeze-thaw cycle	Xenopus laevis

Inhibitors

Inhibitors	Comment	Organism	Structure
antipain	0.05 mM, 35% inhibition	Xenopus laevis
Aprotinin	0.05 mM, 97% inhibition	Xenopus laevis
diisopropyl fluorophosphate	0.05 mM, 37% inhibition. Rapid and irreverible	Xenopus laevis
EDTA	0.05 mM, 90% inhibition	Xenopus laevis
EGTA	0.05 mM, 94% inhibition	Xenopus laevis
guanidine hydrochloride	competitive	Xenopus laevis
leupeptin	0.05 mM, 55% inhibition	Xenopus laevis
additional information	no inhibition by iodoacetamide, E-64, pepstatin or 1,10-phenanthroline	Xenopus laevis
p-aminobenzamidine	competitive	Xenopus laevis
Soybean trypsin inhibitor	0.0025 mM, 84% inhibition	Xenopus laevis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.058	-	Nalpha-tert-butoxycarbonylphenylalanylserylarginyl-7-amido-4-methylcoumarin	-	Xenopus laevis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
secretory granule	oviductal	Xenopus laevis	30141	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	38% stimulation at 10 mM. 11% stimulation at 100 mM	Xenopus laevis
KCl	maximal activity in 200-400 mM solution of monovalent salts is 200% of that in absence of added salt	Xenopus laevis
LiCl	maximal activity in 200-400 mM solution of monovalent salts is 200% of that in absence of added salt	Xenopus laevis
NaCl	maximal activity in 200-400 mM solution of monovalent salts is 200% of that in absence of added salt	Xenopus laevis
NaF	maximal activity in 200-400 mM solution of monovalent salts is 200% of that in absence of added salt	Xenopus laevis
NaNO3	maximal activity in 200-400 mM solution of monovalent salts is 200% of that in absence of added salt	Xenopus laevis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
66000	-	x * 66000, SDS-PAGE under both nonreducing and reducing conditions	Xenopus laevis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
gp43 + H2O	Xenopus laevis	preferential cleavage at Gly-Ser-Arg373-/-glycoprotein gp43 in Xenopus laevis coelemic egg envelope to yield gp41. Processing of gp43 causes physical changes in the egg envelope which occurs during egg transit through the oxiduct	gp41 + ?	-	?

Organism

Organism	UniProt	Comment	Textmining
Xenopus laevis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Xenopus laevis

Source Tissue

Source Tissue	Comment	Organism	Textmining
oviduct	secretory granules	Xenopus laevis	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.53	-	-	Xenopus laevis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
gp43 + H2O	preferential cleavage at Gly-Ser-Arg373-/-glycoprotein gp43 in Xenopus laevis coelemic egg envelope to yield gp41. Processing of gp43 causes physical changes in the egg envelope which occurs during egg transit through the oxiduct	Xenopus laevis	gp41 + ?	-	?
gp43 + H2O	preferential cleavage at Gly-Ser-Arg373-/-glycoprotein gp43 in Xenopus laevis coelemic egg envelope to yield gp41	Xenopus laevis	gp41 + ?	-	?
Nalpha-tert-butoxycarbonyl-Leu-Ser-Thr-Arg-4-methylcoumarin-7-amide + H2O	14% of the activity with Nalpha-tert-butoxycarbonyl-Phe-Ser-Arg-4-methylcoumarin-7-amide	Xenopus laevis	Nalpha-tert-butoxycarbonyl-Leu-Ser-Thr-Arg + 7-amino-4-methylcoumarin	-	?
Nalpha-tert-butoxycarbonyl-Leu-Thr-Arg-4-methylcoumarin-7-amide + H2O	12% of the activity with Nalpha-tert-butoxycarbonyl-Phe-Ser-Arg-4-methylcoumarin-7-amide	Xenopus laevis	Nalpha-tert-butoxycarbonyl-Leu-Thr-Arg + 7-amino-4-methylcoumarin	-	?
Nalpha-tert-butoxycarbonyl-Phe-Ser-Arg-4-methylcoumarin-7-amide + H2O	-	Xenopus laevis	Nalpha-tert-butoxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin	-	?
Nalpha-tert-butoxycarbonylphenylalanylserylarginyl-7-amido-4-methylcoumarin + H2O	-	Xenopus laevis	Nalpha-tert-butoxycarbonylphenylalanylserylarginine + 7-amino-4-methylcoumarin	-	?
Pro-Phe-Arg-4-methylcoumarin-7-amide + H2O	9% of the activity with Nalpha-tert-butoxycarbonyl-Phe-Ser-Arg-4-methylcoumarin-7-amide	Xenopus laevis	Pro-Phe-Arg + 7-amino-4-methylcoumarin	-	?
Pyr-Gly-Arg-4-methylcoumarin-7-amide + H2O	9% of the activity with Nalpha-tert-butoxycarbonyl-Phe-Ser-Arg-4-methylcoumarin-7-amide	Xenopus laevis	Pyr-Gly-Arg + 7-amino-4-methylcoumarin	-	?

Subunits

Subunits	Comment	Organism
?	x * 66000, SDS-PAGE under both nonreducing and reducing conditions	Xenopus laevis

Synonyms

Synonyms	Comment	Organism
Gp43 processing protease	-	Xenopus laevis
oviductal protease	-	Xenopus laevis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.8	-	Nalpha-tert-butoxycarbonylphenylalanylserylarginyl-7-amido-4-methylcoumarin	-	Xenopus laevis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	Nalpha-tert-butoxycarbonylphenylalanylserylarginyl-4-methylcoumarin-7-amide	Xenopus laevis

pH Range

pH Minimum	pH Maximum	Comment	Organism
7.5	8.5	more than 90% of maximal activity at pH 7.5 and at pH 8.5, less than 30% of maximal activity below pH 6.5	Xenopus laevis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0041	-	p-aminobenzamidine	-	Xenopus laevis
0.0075	-	guanidine hydrochloride	-	Xenopus laevis

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Release 2023.1 (January 2023)