Literature summary for 3.4.21.12 extracted from

Kudryakova, I.V.; Gabdulkhakov, A.G.; Tishchenko, S.V.; Lysanskaya, V.Y.; Suzina, N.E.; Tsfasman, I.M.; Afoshin, A.S.; Vasilyeva, N.V.
Structural and functional properties of antimicrobial protein L5 of Lysobacter sp. XL1 (2018), Appl. Microbiol. Biotechnol., 102, 10043-10053 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Lysobacter sp. XL1

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 2.7 M sodium formate and 0.01 M PIPES, at pH 7.0	Lysobacter sp. XL1

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
vesicle	-	Lysobacter sp. XL1	31982	-

Organism

Organism	UniProt	Comment	Textmining
Lysobacter sp. XL1	D2K8B4	VKM B-1576	-

Purification (Commentary)

Purification (Comment)	Organism
His Trap column chromatography and Enrich S column chromatography	Lysobacter sp. XL1

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Abz-Ala-Ala-Phe-4-nitroanilide + H2O	-	Lysobacter sp. XL1	Abz-Ala-Ala-Phe + 4-nitroaniline	-	?
Staphylococcus aureus peptidoglycan + H2O	the enzyme possesses a Gly-Gly endopeptidase activity with respect to staphylococcal peptidoglycan and an amidase that manifests an N-acetylmuramoyl-L-Ala amidase activity with respect to this substrate	Lysobacter sp. XL1	?	-	?

Synonyms

Synonyms	Comment	Organism
alpha-lytic protease	-	Lysobacter sp. XL1
bacteriolytic protease L5	-	Lysobacter sp. XL1
protein L5	-	Lysobacter sp. XL1

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Release 2023.1 (January 2023)