Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.12 extracted from

  • Meyer, J.G.; Kim, S.; Maltby, D.A.; Ghassemian, M.; Bandeira, N.; Komives, E.A.
    Expanding proteome coverage with orthogonal-specificity alpha-lytic proteases (2014), Mol. Cell. Proteomics, 13, 823-835.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.12

Activating Compound

Activating Compound Comment Organism Structure
sodium deoxycholate 196% activity in the presence of 0.1% (w/v) sodium deoxycholate Lysobacter enzymogenes

Protein Variants

Protein Variants Comment Organism
M190A active site mutant Lysobacter enzymogenes

Inhibitors

Inhibitors Comment Organism Structure
guanidine hydrochloride 1% residual activity in the presence of 4 mM guanidine hydrochloride Lysobacter enzymogenes
SDS 42% residual activity in the presence of 1% (w/v) SDS Lysobacter enzymogenes
Urea 12% residual activity in the presence of 4 mM urea Lysobacter enzymogenes

Organism

Organism UniProt Comment Textmining
Lysobacter enzymogenes
-
-
-
Lysobacter enzymogenes 495
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-succinyl-L-Ala-L-Ala-L-Ala 4-nitroanilide + H2O
-
 Lysobacter enzymogenes N-succinyl-L-Ala-L-Ala-L-Ala + 4-nitroaniline
-
 ?
N-succinyl-L-Ala-L-Ala-L-Ala 4-nitroanilide + H2O
-
 Lysobacter enzymogenes 495 N-succinyl-L-Ala-L-Ala-L-Ala + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
alpha-lytic protease
-
 Lysobacter enzymogenes