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Literature summary for 3.4.21.119 extracted from

  • Kapadia, C.; Chang, A.; Sotiropoulou, G.; Yousef, G.M.; Grass, L.; Soosaipillai, A.; Xing, X.; Howarth, D.H.; Diamandis, E.P.
    Human kallikrein 13: production and purification of recombinant protein and monoclonal and polyclonal antibodies, and development of a sensitive and specific immunofluorometric assay (2003), Clin. Chem., 49, 77-86.
    View publication on PubMed
Search for more literature for 3.4.21.119

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-
extracellular enzyme is also secreted into biological fluids Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 secreted enzyme, gel filtration Homo sapiens
50000
-
 x * 50000, recombinant glycosylated enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polypeptide + H2O Homo sapiens
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein enzyme is highly glycosylated Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Pichia pastoris Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
Hydrolyses mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-/- pair in the N-terminus, to yield mature renin enzyme is a serine protease Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
amniotic fluid
-
 Homo sapiens
-
ascites fluid from women with advanced ovarian carcinoma Homo sapiens
-
breast
-
 Homo sapiens
-
cerebrospinal fluid traces Homo sapiens
-
esophagus highest activity in esophagus and tonsil Homo sapiens
-
kidney
-
 Homo sapiens
-
lung
-
 Homo sapiens
-
milk
-
 Homo sapiens
-
additional information fetal tissue pattern is similar to the adult one Homo sapiens
-
ovarian follicle
-
 Homo sapiens
-
prostate
-
 Homo sapiens
-
salivary gland
-
 Homo sapiens
-
seminal plasma
-
 Homo sapiens
-
skin
-
 Homo sapiens
-
testis
-
 Homo sapiens
-
thyroid gland
-
 Homo sapiens
-
tonsil highest activity in esophagus and tonsil Homo sapiens
-
trachea
-
 Homo sapiens
-
ureter
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FSR-amido-4-methylcoumarin + H2O fluorogenic trypsin-like peptide substrate Homo sapiens FSR + 7-amino-4-methylcoumarin
-
 ?
polypeptide + H2O
-
 Homo sapiens peptides
-
 ?
VPR-amido-4-methylcoumarin + H2O fluorogenic trypsin-like peptide substrate Homo sapiens VPR + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, recombinant glycosylated enzyme, SDS-PAGE Homo sapiens
? x * 25000-28000, recombinant deglycosylated enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
K13
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens