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Literature summary for 3.4.21.118 extracted from

  • El Moujahed, A.; Gutman, N.; Brillard, M.; Gauthier, F.
    Substrate specificity of two kallikrein family gene products isolated from the rat submandibular gland (1990), FEBS Lett., 265, 137-140.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by soybean trypsin inhibitor Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
10000
-
x * 10000 + x * 18000, SDS-PAGE Rattus norvegicus
18000
-
x * 10000 + x * 18000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polypeptide + H2O Rattus norvegicus
-
peptides
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
gene KLK8
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys kallikrein k8 exhibit a marked preference for cleavage downstream of Arg residues Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
submaxillary gland
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-Phe-Val-Arg-4-methylcoumarin 7-amide + H2O
-
Rattus norvegicus benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin
-
?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
Rattus norvegicus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
?
additional information kallikrein k8 exhibits a marked preference for cleavage downstream of Arg residues Rattus norvegicus ?
-
?
additional information no activity with tert-butyloxycarbonyl-Leu-Gly-Arg-4-methylcoumarin 7-amide Rattus norvegicus ?
-
?
polypeptide + H2O
-
Rattus norvegicus peptides
-
?
Pro-Phe-Arg-4-methylcoumaryl-7-amide
-
Rattus norvegicus Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
?
tert-butyloxycarbonyl-Phe-Ser-Arg-4-methylcoumaryl-7-amide + H2O no activity Rattus norvegicus tert-butyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
?
tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide + H2O
-
Rattus norvegicus tert-butyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
-
?

Subunits

Subunits Comment Organism
? x * 10000 + x * 18000, SDS-PAGE Rattus norvegicus