Cloned (Comment) | Organism |
---|---|
overexpression of proneuropsin in Spodoptera frugiperda insect cells via baculovirus infection | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(4-amidinohenyl)methanesulfonyl 1-fluoride | 61% inhibition at 1 mM | Mus musculus | |
antipain | 95% inhibition at 0.1 mM | Mus musculus | |
Aprotinin | 65% inhibition at 0.1 mM | Mus musculus | |
benzamidine | 56% inhibition at 1 mM | Mus musculus | |
chymostatin | 80% inhibition at 0.1 mM | Mus musculus | |
diisopropylfluorophosphate | 16% inhibition at 0.1 mM | Mus musculus | |
human alpha1-antichymotrypsin | weak, 16% inhibition at 2 mM | Mus musculus | |
human alpha1-antitrypsin | weak, 20% inhibition at 10 mM | Mus musculus | |
leupeptin | complete inhibition at 0.1 mM | Mus musculus | |
additional information | enzyme is strongly inhibited by low molecular weight protease inhibitors that bind to His and Ser residues in the active center of serine proteases; no or very slight inhibition by pepstatin A, trypsin inhibitor, Ca2+, Mg2+, and metal chelators | Mus musculus | |
trans-epoxysuccinyl-L-leucylamido(4-guanidinobutane) | i.e. E-64, 15% inhibition at 0.1 mM | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
D-Val-Leu-Arg-4-nitroanilide | recombinant enzyme, pH 8.0, 37°C | Mus musculus | |
0.27 | - |
tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide | recombinant enzyme, pH 8.0, 37°C | Mus musculus | |
0.28 | - |
D-Val-Leu-Arg-4-nitroanilide | native enzyme, pH 8.0, 37°C | Mus musculus | |
0.3 | - |
tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide | native enzyme, pH 8.0, 37°C | Mus musculus | |
0.34 | - |
tert-butyloxycarbonyl-Asp-Pro-Arg-4-methylcoumaryl-7-amide | recombinant enzyme, pH 8.0, 37°C | Mus musculus | |
0.5 | - |
tert-butyloxycarbonyl-Phe-Ser-Arg-4-methylcoumaryl-7-amide | recombinant enzyme, pH 8.0, 37°C | Mus musculus | |
0.54 | - |
tert-butyloxycarbonyl-Phe-Ser-Arg-4-methylcoumaryl-7-amide | native enzyme, pH 8.0, 37°C | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
1 * 26000, non-reducing SDS-PAGE | Mus musculus |
26600 | - |
recombinant enzyme, MALDI-TOF MS | Mus musculus |
29000 | - |
recombinant enzyme, gel filtration | Mus musculus |
32000 | - |
1 * 32000, reducing SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
polypeptide + H2O | Mus musculus | enzyme has significant limbic effects by changing the extracellular matrix environment | peptides | - |
? | |
polypeptide + H2O | Mus musculus | involvement in neural plasticity | peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q61955 | adult balb/c mice | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | recombinant enzyme is produced as inactive proform and needs to be processed by an endoprotease, e.g. protease-1, EC 3.4.21.50, or trypsin, EC 3.4.21.4, for activation by specific cleavage of the Lys32-Ile33 bond near the N-terminus | Mus musculus |
Purification (Comment) | Organism |
---|---|
1100fold from brain | Mus musculus |
recombinant from SF21 insect cells | Mus musculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys | enzyme is a trypsin-type serine protease | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
hippocampus | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.23 | - |
purified native enzyme, substrate tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide | Mus musculus |
6.26 | - |
purified recombinant enzyme, substrate tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-Pro-Phe-Arg-4-nitroanilide + H2O | low activity | Mus musculus | acetyl-Pro-Phe-Arg + 4-nitroaniline | - |
? | |
acetyl-Pro-Phe-Arg-4-nitroanilide + H2O | recombinant, not native enzyme | Mus musculus | acetyl-Pro-Phe-Arg + 4-nitroaniline | - |
? | |
D-Val-Leu-Arg-4-nitroanilide + H2O | native and recombinant enzyme | Mus musculus | D-Val-Leu-Arg + 4-nitroaniline | - |
? | |
D-Val-Leu-Lys-4-nitroanilide + H2O | native and recombinant enzyme | Mus musculus | D-Val-Leu-Lys + 4-nitroaniline | - |
? | |
Fibronectin + H2O | recombinant enzyme | Mus musculus | ? | - |
? | |
additional information | recombinant enzyme is produced as inactive proform and needs to be processed by an endoprotease, e.g. protease-1, EC 3.4.21.50, or trypsin, EC 3.4.21.4, for activation by specific cleavage of the Lys32-Ile33 bond near the N-terminus | Mus musculus | ? | - |
? | |
additional information | substrate specificty of native and recombinant enzyme | Mus musculus | ? | - |
? | |
polypeptide + H2O | - |
Mus musculus | peptides | - |
? | |
polypeptide + H2O | enzyme has significant limbic effects by changing the extracellular matrix environment | Mus musculus | peptides | - |
? | |
polypeptide + H2O | involvement in neural plasticity | Mus musculus | peptides | - |
? | |
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-4-methylcoumaryl-7-amide + H2O | recombinant, not native, enzyme | Mus musculus | tert-butyloxycarbonyl-Ala-Gly-Pro-Arg + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-Asp-Pro-Arg-4-methylcoumaryl-7-amide + H2O | recombinant, not native, enzyme | Mus musculus | tert-butyloxycarbonyl-Asp-Pro-Arg + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-Glu-Gly-Arg-4-methylcoumaryl-7-amide + H2O | native and recombinant enzyme | Mus musculus | tert-butyloxycarbonyl-Glu-Gly-Arg + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-Glu-Gly-Arg-4-methylcoumaryl-7-amide + H2O | recombinant, not native, enzyme | Mus musculus | tert-butyloxycarbonyl-Glu-Gly-Arg + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-Glu-Lys-Lys-4-methylcoumaryl-7-amide + H2O | low activity | Mus musculus | tert-butyloxycarbonyl-Glu-Lys-Lys + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-Glu-Lys-Lys-4-methylcoumaryl-7-amide + H2O | recombinant and native enzyme | Mus musculus | tert-butyloxycarbonyl-Glu-Lys-Lys + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-Leu-Arg-Arg-4-methylcoumaryl-7-amide + H2O | low activity | Mus musculus | tert-butyloxycarbonyl-Leu-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-Leu-Arg-Arg-4-methylcoumaryl-7-amide + H2O | recombinant, not native, enzyme | Mus musculus | tert-butyloxycarbonyl-Leu-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-Leu-Thr-Arg-4-methylcoumaryl-7-amide + H2O | low activity | Mus musculus | tert-butyloxycarbonyl-Leu-Thr-Arg + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-Leu-Thr-Arg-4-methylcoumaryl-7-amide + H2O | recombinant, not native, enzyme | Mus musculus | tert-butyloxycarbonyl-Leu-Thr-Arg + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-Phe-Ser-Arg-4-methylcoumaryl-7-amide + H2O | native and recombinant enzyme | Mus musculus | tert-butyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-pyroglutamyl-Gly-Arg-4-methylcoumaryl-7-amide + H2O | recombinant and native enzyme | Mus musculus | tert-butyloxycarbonyl-pyroglutamyl-Gly-Arg + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide + H2O | best substrate | Mus musculus | tert-butyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide + H2O | native and recombinant enzyme | Mus musculus | tert-butyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 26000, non-reducing SDS-PAGE | Mus musculus |
monomer | 1 * 32000, reducing SDS-PAGE | Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant enzyme | Mus musculus |