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Literature summary for 3.4.21.117 extracted from

  • Silva, L.M.; Stoll, T.; Kryza, T.; Stephens, C.R.; Hastie, M.L.; Irving-Rodgers, H.F.; Dong, Y.; Gorman, J.J.; Clements, J.A.
    Mass spectrometry-based determination of kallikrein-related peptidase 7 (KLK7) cleavage preferences and subsite dependency (2017), Sci. Rep., 7, 6789 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.117

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Homo sapiens

Protein Variants

Protein Variants Comment Organism
S195A mutation in catalytic triad, shows residual catalytic activity Homo sapiens
S195A/D102N inactive Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.304
-
 methoxy-succinyl-Arg-Pro-Tyr-7-amido-4-methylcoumarin wild-type, pH 8.0, temperature not specified in the publication Homo sapiens
0.43
-
 methoxy-succinyl-Arg-Pro-Tyr-7-amido-4-methylcoumarin mutant S195A, pH 8.0, temperature not specified in the publication Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P49862
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methoxy-succinyl-Arg-Pro-Tyr-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens methoxy-succinyl-Arg-Pro-Tyr + 7-amino-4-methylcoumarin
-
 ?
additional information KLK7 exerts chymotryptic-like cleavage preferences. KLK7 subsite preferences are also characterised in the P2-P2 region, demonstrating a preference for hydrophobic residues in the non-prime and hydrophilic residues in the prime subsites Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.34
-
 methoxy-succinyl-Arg-Pro-Tyr-7-amido-4-methylcoumarin mutant S195A, pH 8.0, temperature not specified in the publication Homo sapiens
24.5
-
 methoxy-succinyl-Arg-Pro-Tyr-7-amido-4-methylcoumarin wild-type, pH 8.0, temperature not specified in the publication Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.79
-
 methoxy-succinyl-Arg-Pro-Tyr-7-amido-4-methylcoumarin mutant S195A, pH 8.0, temperature not specified in the publication Homo sapiens
80.8
-
 methoxy-succinyl-Arg-Pro-Tyr-7-amido-4-methylcoumarin wild-type, pH 8.0, temperature not specified in the publication Homo sapiens