Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S195A | mutation in catalytic triad, shows residual catalytic activity | Homo sapiens |
S195A/D102N | inactive | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.304 | - |
methoxy-succinyl-Arg-Pro-Tyr-7-amido-4-methylcoumarin | wild-type, pH 8.0, temperature not specified in the publication | Homo sapiens | |
0.43 | - |
methoxy-succinyl-Arg-Pro-Tyr-7-amido-4-methylcoumarin | mutant S195A, pH 8.0, temperature not specified in the publication | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P49862 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methoxy-succinyl-Arg-Pro-Tyr-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | methoxy-succinyl-Arg-Pro-Tyr + 7-amino-4-methylcoumarin | - |
? | |
additional information | KLK7 exerts chymotryptic-like cleavage preferences. KLK7 subsite preferences are also characterised in the P2-P2 region, demonstrating a preference for hydrophobic residues in the non-prime and hydrophilic residues in the prime subsites | Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
methoxy-succinyl-Arg-Pro-Tyr-7-amido-4-methylcoumarin | mutant S195A, pH 8.0, temperature not specified in the publication | Homo sapiens | |
24.5 | - |
methoxy-succinyl-Arg-Pro-Tyr-7-amido-4-methylcoumarin | wild-type, pH 8.0, temperature not specified in the publication | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.79 | - |
methoxy-succinyl-Arg-Pro-Tyr-7-amido-4-methylcoumarin | mutant S195A, pH 8.0, temperature not specified in the publication | Homo sapiens | |
80.8 | - |
methoxy-succinyl-Arg-Pro-Tyr-7-amido-4-methylcoumarin | wild-type, pH 8.0, temperature not specified in the publication | Homo sapiens |