Protein Variants | Comment | Organism |
---|---|---|
additional information | coexpression of enzyme and its substrate pro-sigmaK in Escherichia coli. Enzyme shows abundant and accurate cleavage of substrate. Coexpression of Bacillus subtilis protein BofA in this system leads to formation of a complex of enzyme and BofA and marked inhibition of pro-sigmaK processing. Inhibition may occur by providing H57 of BofA as metal ligand to the catalytic center of enzyme | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pro-sigmaK + H2O | Bacillus subtilis | - |
sigmaK + 20 amino acid peptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
N-terminal cleavage of the pro-form of the sporulation protein sigmaK | enzyme is a metalloprotease with a transmembrane H43EXXH47 motif, E44 promotes nucleophilic attack by a water molecule on the carbonyl atom of the substrate peptide bond | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pro-sigmaK + H2O | - |
Bacillus subtilis | sigmaK + 20 amino acid peptide | - |
? | |
pro-sigmaK + H2O | amino acids 1-126 of substrate pro-sigmaK are sufficient to serve as a substrate | Bacillus subtilis | sigmaK + 20 amino acid peptide | - |
? |