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Literature summary for 3.4.21.115 extracted from
- Characterization of cleavage sites and protease activity in the polyprotein precursor of Japanese marine aquabirnavirus and expression analysis of generated proteins by a VP4 protease activity in four distinct cell lines (2007), Arch. Virol., 152, 1103-1114.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain BL21-DE3 using the pET-41b vector | marine birnavirus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H681F | amino acid substitutions generated in VP4 of MABV by site-directed mutagenesis | marine birnavirus |
| I543G | amino acid substitutions generated in VP4 of MABV by site-directed mutagenesis | marine birnavirus |
| K674D | amino acid substitutions generated in VP4 of MABV by site-directed mutagenesis | marine birnavirus |
| S633P | amino acid substitutions generated in VP4 of MABV by site-directed mutagenesis | marine birnavirus |
| V686Q | amino acid substitutions generated in VP4 of MABV by site-directed mutagenesis | marine birnavirus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 240000 | - |
SDS-PAGE, single band of VP4, same size of VP4 in all analyzed cell lines | marine birnavirus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| marine birnavirus | - |
MABV strain Y-6, isolated from yellowtail Seriola quinqueradiata with ascites | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | specific enzymatic cleavages yield mature virus proteins | marine birnavirus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration, native and recombinant proteins | marine birnavirus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | VP4 protease activity studied in the cell lines CHSE-214 and RSBK-2 that are susceptible to MABV infection, cell lines FHM and EPC resistant to MABV infection included as a control, infection of cell cultures with MABV at a multiplicity of infection of 0.1, respectively | marine birnavirus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | cleavage sites of the MABV polyprotein at the pVP2-VP4 junction determined, VP4 protease activity determined, expression patterns of pVP2, VP4 and VP3 in four infected cell lines shown, VP4 protease activity determined | marine birnavirus | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| infectious pancreatic necrosis birnavirus Vp4 protease | - |
marine birnavirus |
| VP4 | - |
marine birnavirus |
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