Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.112 extracted from

  • Pullikotil, P.; Benjannet, S.; Mayne, J.; Seidah, N.G.
    The proprotein convertase SKI-1/S1P: Alternate translation and subcellular localization (2007), J. Biol. Chem., 282, 27402-27413.
    View publication on PubMed
Search for more literature for 3.4.21.112

Activating Compound

Activating Compound Comment Organism Structure
additional information cellular stress induced by tunicamycin does not regulate the production of St-2 Homo sapiens
N-acetyl-leucinal-leucinal-norleucinal increases the level of St-1 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
full length SKI-1 and its H249A mutant expressed in HEK-293 cells, M19 cells, HuH7 cells and Hep-G2 cells. Wild-type SKI-1 and mutants H249A, L925A and K948A expressed in SRD-12B and CHO-K1 cells. Wild-type SKI-1 or a secretable form of St-1 containing a signal peptide followed by a FLAG tag at the N terminus and a V5 tag at the C terminus overexpressed in CHO-K1 cells. rSt-1 coexpressed with protease inhibitors in CHO-K1 and Neuro-2a cells. Mutants I985A, I989L, M990L, M990A, M990I, Y994A, N995A, and double mutant Y994A/N995A expressed in CHO-K1 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
H249A active site mutant, autocatalytic generation of St-1 is abrogated, St-2 fragment is generated at a level of ca. 15% Homo sapiens
I985A has no effect on generation of St-2 Homo sapiens
I989L results in more than 90% reduction in the generation of St-2 Homo sapiens
K948A shedding site mutant Homo sapiens
L952A shedding site mutant Homo sapiens
M990A fails to generate St-2 Homo sapiens
M990I fails to generate St-2 Homo sapiens
M990L fails to generate St-2 Homo sapiens
additional information in the triple shedding site mutant KLL/A autocatalytic generation of St-1 is abrogated, St-2 fragment is generated at a level of ca. 15% Homo sapiens
N995A has no effect on generation of St-2 Homo sapiens
Y994A has no effect on generation of St-2 Homo sapiens
Y994A/N995A has no effect on generation of St-2 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride inhibits SKI-1 and the autocatalytic generation of St-1 Homo sapiens
brefeldin A abrogates St-2 production Homo sapiens
additional information (Z-LL)2-chloromethylketone and PN1 have no effect on St-2 production. Overexpressed rSt-1 or rSt-2 impede ATF6 processing by SKI-1 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum St-2 production Homo sapiens 5783
-
Golgi apparatus most of membrane-bound SKI-1 and its stumps St-1 and St-2 localize to the Golgi and can enter the endosomal/lysosomal compartments but do not sort to the cell surface Homo sapiens 5794
-
membrane
-
 Homo sapiens 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
8500
-
 St-2 protein, time-of-flight mass spectrometry Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
Hep-G2 cell
-
 Homo sapiens
-
Huh-7 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATF6 precursor + H2O rSt-1 and rSt-2 seem to affect the processing of ATF6 by SKI-1 Homo sapiens nuclear ATF6
-
 ?

Synonyms

Synonyms Comment Organism
S1P
-
 Homo sapiens
SKI-1
-
 Homo sapiens
SKI-1/S1P
-
 Homo sapiens
subtilisin kexin isozyme-1
-
 Homo sapiens