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Literature summary for 3.4.21.112 extracted from

  • Ellermeier, C.D.; Losick, R.
    Evidence for a novel protease governing regulated intramembrane proteolysis and resistance to antimicrobial peptides in Bacillus subtilis (2006), Genes Dev., 20, 1911-1922.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.112

Application

Application Comment Organism
additional information PrsW is responsible for sensing antimicrobial peptides that damage the cell membrane and other agents that cause cell envelope stress Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
prsW+ and mutant E95K expressed in Escherichia coli. PrsW active-site mutants expressed in Bacillus subtilis Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
E75A/E76A is catalytically inactive and traps RsiW in a complex in which the anti-sigma factor is protected from undergoing proteolysis Bacillus subtilis
E95K missense mutant with a a dominant (gain-of-function) mutation. Exhibits heightened resistance to the SdpC toxin in cells lacking the SdpI immunity protein. Is locked in a state that causes constitutive activation of sigmaW Bacillus subtilis
H175A is catalytically inactive and traps RsiW in a complex in which the anti-sigma factor is protected from undergoing proteolysis Bacillus subtilis
additional information prsW insertion/deletion mutant prsW::erm exacerbates the growth defect of an sdpI mutant Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Bacillus subtilis 16020
-

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis PY79
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RsiW + H2O PrsW is both necessary and sufficient for site-1 cleavage of the anti-sigmaW factor RsiW. Site-2 cleavage depends on the prior action of PrsW Bacillus subtilis ?
-
 ?
RsiW + H2O PrsW is both necessary and sufficient for site-1 cleavage of the anti-sigmaW factor RsiW. Site-2 cleavage depends on the prior action of PrsW Bacillus subtilis PY79 ?
-
 ?

Synonyms

Synonyms Comment Organism
protease responsible for activating sigmaW
-
 Bacillus subtilis
PrsW
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 Bacillus subtilis