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Literature summary for 3.4.21.111 extracted from

  • Sakaguchi, M.; Takezawa, M.; Nakazawa, R.; Nozawa, K.; Kusakawa, T.; Nagasawa, T.; Sugahara, Y.; Kawakita, M.
    Role of disulfide bonds in a thermophilic serine protease aqualysin I from Thermus aquaticus YT-1 (2008), J. Biochem., 143, 625-632.
    View publication on PubMed
Search for more literature for 3.4.21.111

Cloned(Commentary)

Cloned (Comment) Organism
wild type and mutant proteins expressed in Escherichia coli TG1 Thermus aquaticus

Protein Variants

Protein Variants Comment Organism
C194S 6% of wild type activity at 40°C, less thermostable than the wild type enzyme, similar secondary structure as the wild type enzyme Thermus aquaticus
C99S 69% of wild type activity at 40°C, almost as stable as the wild type enzyme, similar secondary structure as the wild type enzyme Thermus aquaticus
C99S/C194S 3% of wild type activity at 40°C, less thermostable than the wild type enzyme, similar secondary structure as the wild type enzyme Thermus aquaticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.79
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide wild type enzyme, 40°C, pH 7.5 Thermus aquaticus
1.37
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide C99S mutant enzyme, 40°C, pH 7.5 Thermus aquaticus
2.17
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide C194S P7I mutant enzyme, 40°C, pH 7.5 Thermus aquaticus
3.06
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide C99S/C194S mutant enzyme, 40°C, pH 7.5 Thermus aquaticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 x * 28000, SDS-PAGE Thermus aquaticus

Organism

Organism UniProt Comment Textmining
Thermus aquaticus
-
-
-
Thermus aquaticus YT-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant proteins Thermus aquaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Thermus aquaticus ?
-
 ?
casein + H2O
-
 Thermus aquaticus YT-1 ?
-
 ?
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Thermus aquaticus YT-1 N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 28000, SDS-PAGE Thermus aquaticus

Synonyms

Synonyms Comment Organism
aqualysin I
-
 Thermus aquaticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
 wild type and mutant enzymes with lower activity of the mutant enzymes Thermus aquaticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
86.7
-
 transition temperature of the C194S mutant Thermus aquaticus
86.9
-
 transition temperature of the C99S/C194S mutant Thermus aquaticus
89.8
-
 transition temperature of the C99S mutant Thermus aquaticus
90
-
 half life of wild type enzyme: 45 min, half life of C194S and C99S/C194S mutant enzymes: 10 min Thermus aquaticus
94
-
 transition temperature of the wild type enzyme Thermus aquaticus
100
-
 10 min incubation, wild type retains 40% of activity, complete loss of activity of the C194S and C99S/C194S mutant enzymes Thermus aquaticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide C99S/C194S mutant enzyme, 40°C, pH 7.5 Thermus aquaticus
6.4
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide C194S mutant enzyme, 40°C, pH 7.5 Thermus aquaticus
62
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide C99S mutant enzyme, 40°C, pH 7.5 Thermus aquaticus
80.4
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide wild type enzyme, 40°C, pH 7.5 Thermus aquaticus
91.6
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide wild type enzyme, 40°C, pH 7.5 Thermus aquaticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 wild type Thermus aquaticus