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Literature summary for 3.4.21.110 extracted from
- Processing, stability, and kinetic parameters of C5a peptidase from Streptococcus pyogenes (2002), Eur. J. Biochem., 269, 4839-4851.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Streptococcus pyogenes |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S512A | no autocatalytic cleavage | Streptococcus pyogenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | inhibition at 5 mM | Streptococcus pyogenes |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.36 | - |
SQLRANISHKDMQLGR | pH 7.4, 25°C | Streptococcus pyogenes | |
| 0.936 | - |
VVASQLRANISH | pH 7.4, 25°C | Streptococcus pyogenes | |
| 2 | - |
Ac-SQLRANISH-4-nitroanilide | pH 7.4, 25°C, Km above 2 mM | Streptococcus pyogenes | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chemotaxin C5a | Streptococcus pyogenes | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus pyogenes | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | propeptide is cleaved either via an autocatalytic intramolecular cleavage or by exogenous protease streotopain | Streptococcus pyogenes |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptococcus pyogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ac-SQLRANISH-4-nitroanilide + H2O | weak chromogenic substrate | Streptococcus pyogenes | ? | - |
? | |
| chemotaxin C5a | - |
Streptococcus pyogenes | ? | - |
? | |
| chemotaxin C5a + H2O | cleaves between His67 and Lys68 or Ala58 and Ser59 | Streptococcus pyogenes | ? | - |
? | |
| SQLRANISHKDMQLGR + H2O | good substrate | Streptococcus pyogenes | ? | - |
? | |
| VVASQLRANISH + H2O | - |
Streptococcus pyogenes | VVA + SQLRANISH | - |
? | |
| VVASQLRANISHKDMQLGR + H2O | - |
Streptococcus pyogenes | VVASQLRANISH + KDMQLGR | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 43 | - |
Streptococcus pyogenes |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 43 | increase of temperature from 5°C to 43°C results in 10fold increase in enzyme activity | Streptococcus pyogenes |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
complete inactivation | Streptococcus pyogenes |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
VVASQLRANISH | pH 7.4, 25°C | Streptococcus pyogenes | |
| 1.1 | - |
SQLRANISHKDMQLGR | pH 7.4, 25°C | Streptococcus pyogenes | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
SQLRANISHKDMQLGR as substrate | Streptococcus pyogenes |
| 8.6 | - |
activity against chromogenic substrate Ac-SQLRANISH-4-nitroanilide is 60% higher than at pH 7.4 | Streptococcus pyogenes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
activity against chromogenic substrate Ac-SQLRANISH-4-nitroanilide is 60% higher than at pH 7.4 | Streptococcus pyogenes |
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