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Literature summary for 3.4.21.109 extracted from

  • Ihara, S.; Miyoshi, E.; Nakahara, S.; Sakiyama, H.; Ihara, H.; Akinaga, A.; Honke, K.; Dickson, R.B.; Lin, C.; Taniguchi, N.
    Addition of beta1-6 GlcNAc branching to the oligosaccharide attached to Asn 772 in the serine protease domain of matriptase plays a pivotal role in its stability and resistance against trypsin (2004), Glycobiology, 14, 139-146.
    View publication on PubMed
Search for more literature for 3.4.21.109

Application

Application Comment Organism
additional information resistance to degradation and stabilization of beta1-6GlcNAc matripase are specifically due to the presence of beta1-6GlcNAc branching on the sugar chains, binds strongly to leukoagglutinating phytohemagglutinin, which preferentially recognizes beta1-6 GlcNAc branches of tri- or tetraantennary sugar chains, beta1-6 GlcNAc matriptase is resistant to autodegradation, as well as trypsin digestion, compared with matriptase purified from mock-transfected cells, N-glycosidase-F treatment of beta1-6 GlcNAc matriptase greatly reduces its resistance to degradation Homo sapiens

Protein Variants

Protein Variants Comment Organism
N302Q significant resistance to degradation in the N-acetylglucosminyltransferase V transfectants Homo sapiens
N485Q significant resistance to degradation in the N-acetylglucosminyltransferase V transfectants Homo sapiens
N772Q completely degraded in the N-acetylglucosminyltransferase V transfectants Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from N-acetylglucosminyltransferase V transfectant by immunoaffinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
MKN-45 cell
-
 Homo sapiens
-
additional information COS cell Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gelatin + H2O
-
 Homo sapiens ?
-
 ?
N-Ala-Ala-Ala-Tyr-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens ?
-
 ?
N-succinyl-Ala-Phe-Lys-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens ?
-
 ?
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens ?
-
 ?
N-tert-butoxycarbonyl-gamma-benzyl-Glu-Ala-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens ?
-
 ?
N-tert-butoxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens ?
-
 ?