Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.108 extracted from

  • Bejugam, P.; Kuppili, R.; Singh, N.; Gadewal, N.; Chaganti, L.; Sastry, G.; Bose, K.
    Allosteric regulation of serine protease HtrA2 through novel non-canonical substrate binding pocket (2013), PLoS ONE, 8, e55416.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.108

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Homo sapiens

Protein Variants

Protein Variants Comment Organism
E292A residue involved in most of the peptide interactions, about 30% of wild-type catalytic efficiency Homo sapiens
E296A residue involved in most of the peptide interactions, about 90% of wild-type catalytic efficiency Homo sapiens
F16D residue involved in most of the peptide interactions, almost complete loss of catalytic activity Homo sapiens
G230A residue involved in most of the peptide interactions, about 12% of wild-type catalytic efficiency Homo sapiens
additional information mutant comprising N-terminal and serine protease domains, residues 1–210, displays about 20% of wild-type catalytic efficiency Homo sapiens
N216A/S219A residues involved in most of the peptide interactions, about 33% of wild-type catalytic efficiency Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00302
-
 beta-casein mutant comprising N-terminal and serine protease domains, pH 8.0, 37°C Homo sapiens
0.00459
-
 beta-casein wild-type, pH 8.0, 37°C Homo sapiens
0.00468
-
 beta-casein mutant E296A, pH 8.0, 37°C Homo sapiens
0.00515
-
 beta-casein mutant E292A, pH 8.0, 37°C Homo sapiens
0.00543
-
 beta-casein mutant N216A/S219A, pH 8.0, 37°C Homo sapiens
0.0093
-
 beta-casein mutant F16D, pH 8.0, 37°C Homo sapiens
0.00932
-
 beta-casein mutant G230A, pH 8.0, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O43464
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-casein + H2O
-
 Homo sapiens ?
-
 ?
additional information allostery does regulate HtrA2 activity using a non-canonical selective binding pocket in HtrA2 which initiates signal propagation to the distal active site through a complex allosteric mechanism Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000025
-
 beta-casein mutant F16D, pH 8.0, 37°C Homo sapiens
0.0039
-
 beta-casein mutant comprising N-terminal and serine protease domains, pH 8.0, 37°C Homo sapiens
0.0051
-
 beta-casein mutant G230A, pH 8.0, 37°C Homo sapiens
0.0095
-
 beta-casein mutant E292A, pH 8.0, 37°C Homo sapiens
0.0097
-
 beta-casein mutant N216A/S219A, pH 8.0, 37°C Homo sapiens
0.0187
-
 beta-casein mutant E296A, pH 8.0, 37°C Homo sapiens
0.0204
-
 beta-casein wild-type, pH 8.0, 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0026
-
 beta-casein mutant F16D, pH 8.0, 37°C Homo sapiens
0.54
-
 beta-casein mutant G230A, pH 8.0, 37°C Homo sapiens
1.29
-
 beta-casein mutant comprising N-terminal and serine protease domains, pH 8.0, 37°C Homo sapiens
1.78
-
 beta-casein mutant N216A/S219A, pH 8.0, 37°C Homo sapiens
1.85
-
 beta-casein mutant E292A, pH 8.0, 37°C Homo sapiens
3.99
-
 beta-casein mutant E296A, pH 8.0, 37°C Homo sapiens
4.45
-
 beta-casein wild-type, pH 8.0, 37°C Homo sapiens