Activating Compound | Comment | Organism | Structure |
---|---|---|---|
inhibitor of apoptosis protein | in presence of inhibitor of apoptosis protein, catalytic efficiency increases up to 3fold. In presence of its BIR2 and/or BIR3 domains, catalytic efficiency increases up to 2fold. Interaction allosterically modulates HtrA2 activity, the activation occurs through a series of coordinated structural reorganizations at distal regulatory loops, leading to a population shift towards the relaxed conformer | Homo sapiens | |
PDZ domain G230A | mutation in recognition sequence of the PDZ domain, shows a significant decrease in the catalytic efficiency | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion mutant of the PDZ domain shows a catalytic efficiency 5.5fold less than intact trimeric HtrA2 and the apparent Km and co-operativity are also reduced. Deletion of the PDZ domain possibly affects the initial substrate binding process, allosteric modulation and cleavage, thereby leading to a decrease in co-operativity and catalytic efficiency | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
beta-casein | PDZ domain deletion mutant, K0.5 value, Hill constant 1.7, 37°C, pH 7.8 | Homo sapiens | |
0.0017 | - |
beta-casein | PDZ domain deletion mutant, K0.5 value, Hill constant 1.4, presence of inhibitor of apoptosis domain BIR2, 37°C, pH 7.8 | Homo sapiens | |
0.0017 | - |
beta-casein | PDZ domain deletion mutant, K0.5 value, Hill constant 1.5, presence of inhibitor of apoptosis domain BIR3, 37°C, pH 7.8 | Homo sapiens | |
0.0018 | - |
beta-casein | PDZ domain deletion mutant, K0.5 value, Hill constant 1.5, presence of inhibitor of apoptosis protein, 37°C, pH 7.8 | Homo sapiens | |
0.0023 | - |
beta-casein | wild-type, K0.5 value, Hill constant 1.6, presence of inhibitor of apoptosis domain BIR2, 37°C, pH 7.8 | Homo sapiens | |
0.0023 | - |
beta-casein | wild-type, K0.5 value, Hill constant 1.8, presence of inhibitor of apoptosis domain BIR3, 37°C, pH 7.8 | Homo sapiens | |
0.0023 | - |
beta-casein | wild-type, K0.5 value, Hill constant 2.8, 37°C, pH 7.8 | Homo sapiens | |
0.0027 | - |
beta-casein | wild-type, K0.5 value, Hill constant 1.5, presence of inhibitor of apoptosis protein, 37°C, pH 7.8 | Homo sapiens | |
0.003 | - |
beta-casein | mutant G230A, K0.5 value, presence of inhibitor of apoptosis protein, 37°C, pH 7.8 | Homo sapiens | |
0.0032 | - |
beta-casein | mutant G230A, K0.5 value, 37°C, pH 7.8 | Homo sapiens | |
0.0032 | - |
beta-casein | mutant G230A, K0.5 value, presence of inhibitor of apoptosis domain BIR2, 37°C, pH 7.8 | Homo sapiens | |
0.0035 | - |
beta-casein | mutant G230A, K0.5 value, presence of inhibitor of apoptosis domain BIR3, 37°C, pH 7.8 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O43464 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-casein + H2O | - |
Homo sapiens | ? | - |
? |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
beta-casein | mutant G230A, 37°C, pH 7.8 | Homo sapiens | |
0.26 | - |
beta-casein | mutant G230A, presence of inhibitor of apoptosis domain BIR3, 37°C, pH 7.8 | Homo sapiens | |
0.29 | - |
beta-casein | PDZ domain deletion mutant, Hill constant 1.7, 37°C, pH 7.8 | Homo sapiens | |
0.44 | - |
beta-casein | mutant G230A, presence of inhibitor of apoptosis domain BIR2, 37°C, pH 7.8 | Homo sapiens | |
0.51 | - |
beta-casein | mutant G230A, presence of inhibitor of apoptosis protein, 37°C, pH 7.8 | Homo sapiens | |
0.51 | - |
beta-casein | PDZ domain deletion mutant, Hill constant 1.5, presence of inhibitor of apoptosis domain BIR3, 37°C, pH 7.8 | Homo sapiens | |
0.62 | - |
beta-casein | PDZ domain deletion mutant, Hill constant 1.4, presence of inhibitor of apoptosis domain BIR2, 37°C, pH 7.8 | Homo sapiens | |
0.8 | - |
beta-casein | PDZ domain deletion mutant, Hill constant 1.5, presence of inhibitor of apoptosis protein, 37°C, pH 7.8 | Homo sapiens | |
1.6 | - |
beta-casein | wild-type, Hill constant 2.8, 37°C, pH 7.8 | Homo sapiens | |
3.1 | - |
beta-casein | wild-type, Hill constant 1.8, presence of inhibitor of apoptosis domain BIR3, 37°C, pH 7.8 | Homo sapiens | |
3.8 | - |
beta-casein | wild-type, Hill constant 1.6, presence of inhibitor of apoptosis domain BIR2, 37°C, pH 7.8 | Homo sapiens | |
4.7 | - |
beta-casein | wild-type, Hill constant 1.5, presence of inhibitor of apoptosis protein, 37°C, pH 7.8 | Homo sapiens |