Cloned (Comment) | Organism |
---|---|
recombinantly expressed | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S306A | mutant lacks proteolytic activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ucf-101 | proteolysis of Wilms'tumor suppressor protein WT1 is prevented | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | HtrA2 fails to cleave the Wilms' tumor suppressor 1 cofactors Par-4 and BASP1 | ? | - |
? | |
Wilms' tumor suppressor 1 + H2O | Homo sapiens | HtrA2 cleaves at, at least, two specific sites. Wilms' tumor suppressor 1 degradation by HtrA2 reveals two major proteolytic products of 20000 Da and 15000 Da, but the same samples immunoblotted with N-Ter antibody reveals major fragment sizes of 35000 Da and 30000 Da. The potential cleavage site that produces the 20000 Da fragment is at Val286, and the potential cleavage site producing the 15000 Da fragment is at Leu320 | ? | - |
? | |
Wilms' tumor suppressor protein WT1 + H2O | Mus musculus | - |
? | - |
? | |
Wilms' tumor suppressor protein WT1 + H2O | Homo sapiens | using a yeat two-hybrid assay WT1 is identified as a binding partner of HtrA2/Omi. HtrA2/Omi cleaves WT1 at multiple binding sites following the treatment of cells with cytotoxic drugs | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryonic fibroblast cell line | - |
Mus musculus | - |
K-562 cell | - |
Homo sapiens | - |
NCI-H1299 cell | - |
Homo sapiens | - |
U2-OS cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | HtrA2 fails to cleave the Wilms' tumor suppressor 1 cofactors Par-4 and BASP1 | Homo sapiens | ? | - |
? | |
Wilms' tumor suppressor 1 + H2O | HtrA2 cleaves at, at least, two specific sites. Wilms' tumor suppressor 1 degradation by HtrA2 reveals two major proteolytic products of 20000 Da and 15000 Da, but the same samples immunoblotted with N-Ter antibody reveals major fragment sizes of 35000 Da and 30000 Da. The potential cleavage site that produces the 20000 Da fragment is at Val286, and the potential cleavage site producing the 15000 Da fragment is at Leu320 | Homo sapiens | ? | - |
? | |
Wilms' tumor suppressor protein WT1 + H2O | - |
Mus musculus | ? | - |
? | |
Wilms' tumor suppressor protein WT1 + H2O | using a yeat two-hybrid assay WT1 is identified as a binding partner of HtrA2/Omi. HtrA2/Omi cleaves WT1 at multiple binding sites following the treatment of cells with cytotoxic drugs | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
high temperature requirement protein A2 | - |
Homo sapiens |
HtrA2/Omi | - |
Mus musculus |
HtrA2/Omi | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mus musculus |
7.5 | - |
assay at | Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | ablation of HtrA2 activity using RNAi in the human osteosarcoma U2OS cells prevents the cleavage of Wilms'tumor suppressor protein WT1 under apoptotic conditions | down |
General Information | Comment | Organism |
---|---|---|
malfunction | in mouse embryonic HtrA2/omi knockout fibroblast cells the apoptosis-dependent cleavage of Wilms'tumor suppressor protein WT1 is prevented and expression of WT1 is upregulated | Mus musculus |
physiological function | HtrA2/Omi cleave Wilms'tumor suppressor protein WT1 which causes the removal of WT1 from its binding sites at gene promoters, leading to alterations in gene regulation that enhance apoptosis | Homo sapiens |
physiological function | proteolysis of Wilms' tumor suppressor 1 by HtrA2 causes the removal of WT1 from its binding sites at gene promoters, leading to alterations in gene regulation that enhance apoptosis | Homo sapiens |