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Literature summary for 3.4.21.108 extracted from

  • Hartkamp, J.; Carpenter, B.; Roberts, S.G.
    The Wilms tumor suppressor protein WT1 is processed by the serine protease HtrA2/Omi (2010), Mol. Cell, 37, 159-171.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.108

Cloned(Commentary)

Cloned (Comment) Organism
recombinantly expressed Homo sapiens

Protein Variants

Protein Variants Comment Organism
S306A mutant lacks proteolytic activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
ucf-101 proteolysis of Wilms'tumor suppressor protein WT1 is prevented Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens HtrA2 fails to cleave the Wilms' tumor suppressor 1 cofactors Par-4 and BASP1 ?
-
 ?
Wilms' tumor suppressor 1 + H2O Homo sapiens HtrA2 cleaves at, at least, two specific sites. Wilms' tumor suppressor 1 degradation by HtrA2 reveals two major proteolytic products of 20000 Da and 15000 Da, but the same samples immunoblotted with N-Ter antibody reveals major fragment sizes of 35000 Da and 30000 Da. The potential cleavage site that produces the 20000 Da fragment is at Val286, and the potential cleavage site producing the 15000 Da fragment is at Leu320 ?
-
 ?
Wilms' tumor suppressor protein WT1 + H2O Mus musculus
-
 ?
-
 ?
Wilms' tumor suppressor protein WT1 + H2O Homo sapiens using a yeat two-hybrid assay WT1 is identified as a binding partner of HtrA2/Omi. HtrA2/Omi cleaves WT1 at multiple binding sites following the treatment of cells with cytotoxic drugs ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
embryonic fibroblast cell line
-
 Mus musculus
-
K-562 cell
-
 Homo sapiens
-
NCI-H1299 cell
-
 Homo sapiens
-
U2-OS cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information HtrA2 fails to cleave the Wilms' tumor suppressor 1 cofactors Par-4 and BASP1 Homo sapiens ?
-
 ?
Wilms' tumor suppressor 1 + H2O HtrA2 cleaves at, at least, two specific sites. Wilms' tumor suppressor 1 degradation by HtrA2 reveals two major proteolytic products of 20000 Da and 15000 Da, but the same samples immunoblotted with N-Ter antibody reveals major fragment sizes of 35000 Da and 30000 Da. The potential cleavage site that produces the 20000 Da fragment is at Val286, and the potential cleavage site producing the 15000 Da fragment is at Leu320 Homo sapiens ?
-
 ?
Wilms' tumor suppressor protein WT1 + H2O
-
 Mus musculus ?
-
 ?
Wilms' tumor suppressor protein WT1 + H2O using a yeat two-hybrid assay WT1 is identified as a binding partner of HtrA2/Omi. HtrA2/Omi cleaves WT1 at multiple binding sites following the treatment of cells with cytotoxic drugs Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
high temperature requirement protein A2
-
 Homo sapiens
HtrA2/Omi
-
 Mus musculus
HtrA2/Omi
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Mus musculus
7.5
-
 assay at Homo sapiens

Expression

Organism Comment Expression
Homo sapiens ablation of HtrA2 activity using RNAi in the human osteosarcoma U2OS cells prevents the cleavage of Wilms'tumor suppressor protein WT1 under apoptotic conditions down

General Information

General Information Comment Organism
malfunction in mouse embryonic HtrA2/omi knockout fibroblast cells the apoptosis-dependent cleavage of Wilms'tumor suppressor protein WT1 is prevented and expression of WT1 is upregulated Mus musculus
physiological function HtrA2/Omi cleave Wilms'tumor suppressor protein WT1 which causes the removal of WT1 from its binding sites at gene promoters, leading to alterations in gene regulation that enhance apoptosis Homo sapiens
physiological function proteolysis of Wilms' tumor suppressor 1 by HtrA2 causes the removal of WT1 from its binding sites at gene promoters, leading to alterations in gene regulation that enhance apoptosis Homo sapiens