Application | Comment | Organism |
---|---|---|
additional information | simple and rapid strategy for molecular cloning using a gel-free and antibiotic selection method, which allows for the complete elimination of DNA extraction by gel electrophoresis, and thus has several advantages over gel-based cloning methods, including a cloning efficiency that is approximately 10times higher due to the prevention of ethidium bromide ultraviolet-induced DNA damage and contamination with ligase inhibitors, the amount of plasmid DNA required is approximately five times less, and cloning time is several hours less | Mus musculus |
Cloned (Comment) | Organism |
---|---|
into plasmid pEGFPN3 and plasmid pGEX-4T1-FLAG. Expression of GST-HtrA2 fusion protein in BL21 cells and in HEK-293 cells | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
by immunoblotting | Mus musculus |
61790 | - |
GST-HtrA2, calculated by the Compute pI/Mw method at the ExPASy proteomics server of the Swiss Institute of Bioinformatics | Mus musculus |
63000 | - |
GST-HtrA2, by immunoblotting | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity purified | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-casein + H2O | cleaved to approximately 50% by GST-HtrA2 after a cleavage reaction for 1 h | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HtrA2 serine protease | - |
Mus musculus |