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Literature summary for 3.4.21.107 extracted from

  • deRegt, A.; Kim, S.; Sohn, J.; Grant, R.; Baker, T.; Sauer, R.
    A conserved activation cluster is required for allosteric communication in HtrA-family proteases (2015), Structure, 23, 517-526.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.107

Activating Compound

Activating Compound Comment Organism Structure
Tyr-Tyr-Phe tripeptide stimulates activity Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
D221A mutant displays reduced peptide Tyr-Tyr-Phe-stimulated cleavage activity Escherichia coli
D221A/H198P mutant displays more than 65% of peptide Tyr-Tyr-Phe-stimulated cleavage activity of mutant H198P Escherichia coli
F220A mutant has almost no cleavage activity Escherichia coli
F234A mutagenic analysis of allosteric activation, less than 2% of peptide Tyr-Tyr-Phe-stimulated wild-type activity Escherichia coli
H198P mutation stabilizes active DegS and increases the proteolytic activity of otherwise wild-type DegSabout 6-fold under assay conditions Escherichia coli
I179A mutant displays reduced peptide Tyr-Tyr-Phe-stimulated cleavage activity Escherichia coli
I179A/H198P D221A mutant displays more than 65% of peptide Tyr-Tyr-Phe-stimulated cleavage activity of mutant H198P Escherichia coli
I232A mutagenic analysis of allosteric activation, less than 2% of peptide Tyr-Tyr-Phe-stimulated wild-type activity Escherichia coli
L164A mutagenic analysis of allosteric activation, less than 2% of peptide Tyr-Tyr-Phe-stimulated wild-type activity Escherichia coli
N197A mutagenic analysis of allosteric activation, less than 2% of peptide Tyr-Tyr-Phe-stimulated wild-type activity Escherichia coli
P161A mutagenic analysis of allosteric activation, less than 2% of peptide Tyr-Tyr-Phe-stimulated wild-type activity Escherichia coli
Q187A mutant displays reduced peptide Tyr-Tyr-Phe-stimulated cleavage activity Escherichia coli
Q187A/H198P mutant displays more than 65% of peptide Tyr-Tyr-Phe-stimulated cleavage activity of mutant H198P Escherichia coli
Q191A mutagenic analysis of allosteric activation, less than 2% of peptide Tyr-Tyr-Phe-stimulated wild-type activity Escherichia coli
R178A mutant has almost no cleavage activity Escherichia coli
R178A/H198P 9-24% of wild-type activity Escherichia coli
T167V mutagenic analysis of allosteric activation, less than 2% of peptide Tyr-Tyr-Phe-stimulated wild-type activity Escherichia coli
T169A mutagenic analysis of allosteric activation, less than 2% of peptide Tyr-Tyr-Phe-stimulated wild-type activity Escherichia coli
Y162A mutagenic analysis of allosteric activation, less than 2% of peptide Tyr-Tyr-Phe-stimulated wild-type activity Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AEE3
-
-

Synonyms

Synonyms Comment Organism
DegS
-
 Escherichia coli