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Literature summary for 3.4.21.107 extracted from

  • Baek, K.T.; Vegge, C.S.; Skorko-Glonek, J.; Brondsted, L.
    Different contributions of HtrA protease and chaperone activities to Campylobacter jejuni stress tolerance and physiology (2011), Appl. Environ. Microbiol., 77, 57-66.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.107

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells (wild type enzyme) and ion Escherichia coli BL20 cells (mutant enzyme S197A) Campylobacter jejuni

Protein Variants

Protein Variants Comment Organism
S197A protease-negative mutant protein with decreased chaperone activity compared to the wild type Campylobacter jejuni

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Campylobacter jejuni
-
-

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
-
-
-
Campylobacter jejuni NCTC 11168
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-casein + H2O
-
 Campylobacter jejuni ?
-
 ?
beta-casein + H2O
-
 Campylobacter jejuni NCTC 11168 ?
-
 ?

Synonyms

Synonyms Comment Organism
HtrA
-
 Campylobacter jejuni

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Campylobacter jejuni

Expression

Organism Comment Expression
Campylobacter jejuni protease activity stimulates HtrA production and oligomer formation up

General Information

General Information Comment Organism
physiological function HtrA displays both temperature-dependent chaperone and protease activities. The HtrA chaperone activity is sufficient for growth at high temperature or under oxidative stress (in the presence of H2O2, cumene hydroperoxide or paraquat) whereas the HtrA protease activity is essential only under conditions close to the growth limit for Campylobacter jejuni. However, the protease activity is required to prevent induction of the cytoplasmic heat shock response even under optimal growth conditions. HtrA may protect oxidatively damaged proteins Campylobacter jejuni