Literature summary for 3.4.21.107 extracted from

Chamberland, A.; Wang, E.; Jones, A.R.; Collins-Racie, L.A.; LaVallie, E.R.; Huang, Y.; Liu, L.; Morris, E.A.; Flannery, C.R.; Yang, Z.
Identification of a novel HtrA1-susceptible cleavage site in human aggrecan: evidence for the involvement of HtrA1 in aggrecan proteolysis in vivo (2009), J. Biol. Chem., 284, 27352-27359.

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Activating Compound

Activating Compound	Comment	Organism	Structure
CPII	cleavage of aggrecan by HtrA1 is strongly enhanced by HtrA1 agonists such as CPII, a C-terminal hexapeptide derived from the C-propeptide of procollagen IIalpha1	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
S328A	active site mutant	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
aggrecan + H2O	Homo sapiens	the HtrA1-specific cleavage site is VQTV3562357TWPD within the interglobular domain of aggrecan	aggrecan fragments	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
articular cartilage	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
aggrecan + H2O	the HtrA1-specific cleavage site is VQTV3562357TWPD within the interglobular domain of aggrecan	Homo sapiens	aggrecan fragments	-	?

Synonyms

Synonyms	Comment	Organism
HtrA1	-	Homo sapiens
PRSS11	-	Homo sapiens

Expression

Organism	Comment	Expression
Homo sapiens	HtrA1 levels are highly elevated (about 7.8fold) in osteoarthritic cartilage	up

General Information

General Information	Comment	Organism
physiological function	HtrA1 is a critical protease involved in proteoglycan turnover and cartilage degradation during degenerative joint disease	Homo sapiens

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Release 2023.1 (January 2023)